PNP 水凝胶可预防眼部碱烧伤后小鼠出现睑球粘连。
PNP Hydrogel Prevents Formation of Symblephara in Mice After Ocular Alkali Injury.
机构信息
Department of Ophthalmology, Stanford University School of Medicine, Palo Alto, CA, USA.
Department of Materials Science and Engineering, Stanford University, Palo Alto, CA, USA.
出版信息
Transl Vis Sci Technol. 2022 Feb 1;11(2):31. doi: 10.1167/tvst.11.2.31.
PURPOSE
To create an alkali injury symblephara mouse model to study conjunctival fibrosis pathophysiology and test polymer nanoparticle (PNP) hydrogel as a preventative therapeutic.
METHODS
Mice were injured using NaOH-soaked filter paper to determine the optimal NaOH concentration to induce the formation of symblephara. Injured mice were observed for 7 days to detect the formation of symblephara. Forniceal shortening observed on hematoxylin and eosin (H&E)-stained tissue sections was used as a symblephara marker. Alpha-smooth muscle actin (α-SMA) expression, Masson's trichrome assay, and periodic acid-Schiff (PAS) staining were used to determine myofibroblast expression, collagen deposition, and goblet cell integrity. PNP hydrogel, with multivalent, noncovalent interactions between modified biopolymers and nanoparticles, was applied immediately after alkali injury to determine its ability to prevent the formation of symblephara.
RESULTS
Forniceal shortening was observed in H&E images with 1N NaOH for 2 minutes after 7 days without globe destruction. PNP hydrogel prevented forniceal shortening after alkali injury as observed by H&E histology. α-SMA expression and collagen deposition in eye tissue sections were increased in the fornix after injury with 1N NaOH compared with uninjured controls. PNP hydrogel treatment immediately after injury reduced α-SMA expression and collagen deposition in the forniceal region. Mucin-secreting goblet cells stained with PAS were significantly lower in alkali-injured and PNP hydrogel-treated conjunctivas than in uninjured control conjunctivas.
CONCLUSIONS
We observed that 1N NaOH for 2 minutes induced maximal forniceal shortening and symblephara in mice. PNP hydrogel prevented forniceal shortening and conjunctival fibrosis after injury. This first murine model for symblephara will be useful to study fibrosis pathophysiology after conjunctival injury and to determine therapeutic targets for cicatrizing diseases.
TRANSLATIONAL RELEVANCE
This mouse model of symblephara can be useful for studying conjunctival scarring disease pathophysiology and preventative therapeutics. We tested PNP hydrogel, which prevented the formation of symblephara after injury.
目的
建立碱烧伤兔唇模型，研究结膜纤维化发病机制，并测试聚合物纳米颗粒（PNP）水凝胶作为预防治疗药物。
方法
用浸有 NaOH 的滤纸对小鼠进行损伤，以确定诱导兔唇形成的最佳 NaOH 浓度。观察受伤小鼠 7 天，以检测兔唇的形成。苏木精和伊红（H&E）染色组织切片上观察到的穹窿缩短被用作兔唇的标志物。α-平滑肌肌动蛋白（α-SMA）表达、Masson 三色染色和过碘酸希夫（PAS）染色用于确定肌成纤维细胞表达、胶原沉积和杯状细胞完整性。PNP 水凝胶通过修饰生物聚合物和纳米颗粒之间的多价非共价相互作用，在碱损伤后立即应用，以确定其预防兔唇形成的能力。
结果
用 1N NaOH 处理 2 分钟后，7 天后在 H&E 图像中观察到穹窿缩短，且眼球未受损。H&E 组织学观察到 PNP 水凝胶可预防碱损伤后的穹窿缩短。与未受伤对照相比，用 1N NaOH 损伤后，眼组织切片中穹窿的 α-SMA 表达和胶原沉积增加。损伤后立即用 PNP 水凝胶治疗可减少穹窿区域的 α-SMA 表达和胶原沉积。与未受伤对照相比，用碱损伤和 PNP 水凝胶处理的结膜中，PAS 染色的分泌粘蛋白的杯状细胞明显减少。
结论
我们观察到，用 1N NaOH 处理 2 分钟可诱导小鼠最大的穹窿缩短和兔唇。PNP 水凝胶可预防损伤后穹窿缩短和结膜纤维化。这种兔唇的首个模型将有助于研究结膜损伤后的纤维化发病机制，并确定瘢痕性疾病的治疗靶点。
翻译后记
这段译文整体比较简单，涉及到一些生物医学领域的术语，如“symblephara”（兔唇）、“forniceal shortening”（穹窿缩短）、“myofibroblast”（肌成纤维细胞）等。在翻译过程中，需要确保术语的准确性和一致性。
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