Olofsson P
Eur Surg Res. 1986;18(2):112-21. doi: 10.1159/000128513.
A quantitative spectrophotometric assay for endotoxin, utilizing Limulus amebocyte lysate and a chromogenic peptide substrate, was standardized for the application on lymph and plasma from the rat. The influence of inhibitors and unspecific amidolytic activity, the optimal incubation times, the adhesion of endotoxin to platelets and glass surface, the variation in endotoxin standard as well as the effects of cold storage on endotoxin activity were settled. In vitro added as well as endogenously derived endotoxin was studied. The method was found to be reliable when in practical use on these media.
一种利用鲎试剂和显色肽底物的内毒素定量分光光度测定法，已针对大鼠淋巴液和血浆进行了标准化应用。确定了抑制剂和非特异性酰胺分解活性的影响、最佳孵育时间、内毒素与血小板及玻璃表面的黏附、内毒素标准品的变化以及冷藏对内毒素活性的影响。研究了体外添加的内毒素和内源性内毒素。结果发现该方法在实际应用于这些介质时是可靠的。
