Microbiology and Immunology Unit, Department of Pathology, College of Medicine, Jouf University, Sakaka City Al-Jouf, Saudi Arabia.
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt.
J Infect Dev Ctries. 2022 Jan 31;16(1):125-133. doi: 10.3855/jidc.14885.
Tuberculosis has been a concern of healthcare professionals due to the serious threats it poses on public health safety. However, regardless all the efforts, no appropriate goals for immunological diagnosis or tuberculosis treatment were established. Toll-like receptor 2 is one of the toll-like receptors, which plays a fundamental role in recognizing and hosting defense against Mycobacterium tuberculosis infection. Toll-like receptor 2's genetic polymorphism (arginine-to-glutamine substitution at residue 753 (Arg753Gln)) was linked to negative effects on the function of Toll-like receptor 2 which, in turn, impacts the body's resistance or susceptibility to tuberculosis. The current study aimed at investigating the single Arg753Gln nucleotide polymorphism of the Toll-like receptor 2 gene in patients with tuberculosis infection versus a sample of healthy subjects as controls.
A comparative study was conducted to investigate Toll-like receptor 2 polymorphism of the single nucleotide gene Arg753Gln in 30 patients with pulmonary tuberculosis and compare their results with other 20 healthy controls matched by age and sex.
TLR-2-Arg polymorphism allele A occurred in 36.7% of the patient group. Homozygous carriers of allele A/A polymorphism occurred in 13.4% compared to 5% among controls, while GA genotype was found in 23.3% among the study group and 10% among controls. The association between GA genotype and pulmonary tuberculosis was found statistically significant (p = 0.002) than other genotypes. Allele frequency for both G and A were (p =0.002) in patient groups and (p =0.000) among the control group.
TLR-2 Arg753Gln polymorphisms may have a crucial role in pulmonary tuberculosis susceptibility among Egyptian patients.
由于结核病对公众健康安全构成的严重威胁，一直以来都是医疗保健专业人员关注的焦点。然而，尽管付出了所有努力，仍未确定针对结核病免疫诊断或治疗的适当目标。Toll 样受体 2 是 Toll 样受体之一，在识别和宿主防御结核分枝杆菌感染方面发挥着重要作用。Toll 样受体 2 的基因多态性（第 753 位精氨酸到谷氨酰胺取代（Arg753Gln））与 Toll 样受体 2 功能的负面影响有关，进而影响机体对结核病的抵抗力或易感性。本研究旨在调查结核病感染患者与健康对照组 Toll 样受体 2 基因单核苷酸 Arg753Gln 多态性。
采用病例对照研究方法，对 30 例肺结核患者 Toll 样受体 2 基因单核苷酸 Arg753Gln 多态性进行检测，并与同期 20 例健康体检者进行比较。
TLR-2-Arg 多态性等位基因 A 在患者组中的发生率为 36.7%。与对照组的 5%相比，纯合子携带者 A/A 多态性发生率为 13.4%，而 GA 基因型在研究组中为 23.3%，在对照组中为 10%。GA 基因型与肺结核之间存在统计学显著相关性（p = 0.002），与其他基因型相比。患者组和对照组的 G 和 A 等位基因频率均有统计学差异（p =0.002）和（p =0.000）。
TLR-2 Arg753Gln 多态性可能在埃及患者的肺结核易感性中起关键作用。
