Lam Ho Yin Pekkle, Hung Meng-Yun, Liang Ting-Ruei, Peng Shih-Yi
Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan.
Department of Biochemistry, School of Medicine, Tzu Chi University, Hualien, Taiwan.
Iran J Pharm Res. 2021 Fall;20(4):300-314. doi: 10.22037/ijpr.2021.115154.15225.
Currently, there are no effective treatments for liver diseases. Treatment usually involves controlling complications and supportive care. As liver injuries also affect other organs such as the spleen, kidney, and brain due to their anatomical and physiological relationships, finding an effective treatment is urgently needed. This research aimed to evaluate the therapeutic effect of Schisandrin B (Sch B) in the liver and other organs in thioacetamide (TAA)-intoxicated mice. In this study, mice were exposed to a single intraperitoneal injection of 200 mg/kg TAA to induce hepatitis. Following Sch B (20 mg/kg/day, 28 consecutive days) treatment, biochemistry analysis and histopathological examination of different organs were performed, in addition to western blotting and flow cytometry to evaluate the involvement of inflammasomes and apoptotic proteins. Our results showed that administration of Sch B protected against TAA-induced damages, and it disparately affected inflammasome activation and apoptosis in different organs. Furthermore, Sch B treatment improved organ function, as indicated by the improvement of serum biochemical parameters. Collectively, our findings reveal a beneficial effect of Sch B on different organ damages in mice intoxicated with TAA.
目前，尚无针对肝脏疾病的有效治疗方法。治疗通常包括控制并发症和支持性护理。由于肝脏与脾脏、肾脏和大脑等其他器官在解剖学和生理学上存在关联，肝脏损伤也会影响这些器官，因此迫切需要找到一种有效的治疗方法。本研究旨在评估五味子乙素（Sch B）对硫代乙酰胺（TAA）中毒小鼠肝脏及其他器官的治疗效果。在本研究中，给小鼠腹腔注射一次200 mg/kg的TAA以诱导肝炎。在Sch B（20 mg/kg/天，连续28天）治疗后，除了进行蛋白质印迹法和流式细胞术以评估炎性小体和凋亡蛋白的参与情况外, 还对不同器官进行了生化分析和组织病理学检查。我们的结果表明，给予Sch B可预防TAA诱导的损伤，并且它对不同器官中炎性小体的激活和细胞凋亡有不同的影响。此外，Sch B治疗改善了器官功能，血清生化参数的改善表明了这一点。总的来说，我们的研究结果揭示了Sch B对TAA中毒小鼠不同器官损伤具有有益作用。
