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大肠杆菌flaA(cheC)基因座序列及一个新基因的发现。

Sequence of the flaA (cheC) locus of Escherichia coli and discovery of a new gene.

作者信息

Kuo S C, Koshland D E

出版信息

J Bacteriol. 1986 Jun;166(3):1007-12. doi: 10.1128/jb.166.3.1007-1012.1986.

DOI:10.1128/jb.166.3.1007-1012.1986
PMID:3519573
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC215225/
Abstract

The flaA (cheC) locus from Escherichia coli is important in controlling the rotational direction of flagella during chemotaxis. The locus was sequenced, and a site of transcriptional initiation was determined. Two reading frames, flaAI and flaAII, span the locus. flaAII corresponds to certain flaA and cheC mutations, and has some unusual features in the predicted secondary structure. flaAI, however, has not been identified previously, but a flaAI deletion, which produced a truncated FlaAI peptide in minicells, clearly identified the FlaAI protein.

摘要

来自大肠杆菌的flaA(cheC)基因座在趋化作用期间控制鞭毛的旋转方向方面很重要。对该基因座进行了测序,并确定了转录起始位点。两个阅读框flaAI和flaAII跨越该基因座。flaAII与某些flaA和cheC突变相对应,并且在预测的二级结构中具有一些不寻常的特征。然而,flaAI以前尚未被鉴定出来,但一个flaAI缺失(在微细胞中产生截短的FlaAI肽)明确鉴定出了FlaAI蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4599/215225/5fb50422db27/jbacter00211-0323-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4599/215225/14635833fb45/jbacter00211-0323-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4599/215225/5fb50422db27/jbacter00211-0323-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4599/215225/14635833fb45/jbacter00211-0323-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4599/215225/5fb50422db27/jbacter00211-0323-b.jpg

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