Musselman Catherine A, Kutateladze Tatiana G
Department of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, United States.
Department of Pharmacology, University of Colorado School of Medicine, Aurora, Colorado 80045, United States.
ACS Chem Biol. 2022 Mar 18;17(3):495-502. doi: 10.1021/acschembio.1c00954. Epub 2022 Feb 23.
The formation of chromatin not only compacts the eukaryotic genome into the nucleus but also provides a mechanism for the regulation of all DNA templated processes. Spatial and temporal modulation of the chromatin structure is critical in such regulation and involves fine-tuned functioning of the basic subunit of chromatin, the nucleosome. It has become apparent that the nucleosome is an inherently dynamic system, but characterization of these dynamics at the atomic level has remained challenging. NMR spectroscopy is a powerful tool for investigating the conformational ensemble and dynamics of proteins and protein complexes, and recent advances have made the study of large systems possible. Here, we review recent studies which utilize NMR spectroscopy to uncover the atomic level conformation and dynamics of the nucleosome and provide a better understanding of the importance of these dynamics in key regulatory events.
染色质的形成不仅将真核生物基因组压缩进细胞核，还为所有以DNA为模板的过程提供了一种调控机制。染色质结构的时空调节在这种调控中至关重要，并且涉及染色质基本亚基核小体的精细运作。很明显，核小体是一个内在的动态系统，但在原子水平上表征这些动态仍然具有挑战性。核磁共振波谱是研究蛋白质和蛋白质复合物的构象集合及动态的有力工具，最近的进展使研究大型系统成为可能。在这里，我们综述了最近利用核磁共振波谱揭示核小体原子水平构象和动态的研究，并更好地理解了这些动态在关键调控事件中的重要性。
