Tomasek J J, Schultz R J, Episalla C W, Newman S A
J Hand Surg Am. 1986 May;11(3):365-71. doi: 10.1016/s0363-5023(86)80143-5.
The cytoskeleton and the extracellular matrix of myofibroblasts in nodules from Dupuytren's diseased palmar fascia were examined by indirect immunofluorescence. Primary antibodies used as probes of these tissue compartments were directed against (1) smooth muscle myosin, (2) nonmuscle myosin--components of the cytoplasmic contractile apparatus in smooth muscle and nonmuscle cells, respectively, (3) laminin, and (4) fibronectin--extracellular glycoproteins mediating cell-matrix attachment in smooth muscle and nonmuscle fibroblastic cells, respectively. The Dupuytren's nodular cells stained for nonmuscle myosin and fibronectin but not for smooth muscle myosin or laminin; this indicated that, at the level of biochemical differentiation, these cells are a nonmuscle type. Staining for fibronectin between nodular cells was dramatically increased over that seen between fibroblasts of the normal palmar fascia. Because of the non-muscle nature of the distinctive contractile cell type of the Dupuytren's nodule, we suggest that the term myofibroblast should be considered a misnomer when applied to this pathogenic cell type.
通过间接免疫荧光法对掌腱膜Dupuytren病结节中的肌成纤维细胞的细胞骨架和细胞外基质进行了检测。用作这些组织成分探针的一抗分别针对（1）平滑肌肌球蛋白、（2）非肌肉肌球蛋白——分别为平滑肌和非肌肉细胞中细胞质收缩装置的成分、（3）层粘连蛋白，以及（4）纤连蛋白——分别介导平滑肌和非肌肉成纤维细胞中细胞-基质附着的细胞外糖蛋白。Dupuytren病结节细胞对非肌肉肌球蛋白和纤连蛋白呈阳性染色，但对平滑肌肌球蛋白或层粘连蛋白呈阴性染色；这表明，在生化分化水平上，这些细胞属于非肌肉类型。结节细胞之间纤连蛋白的染色明显高于正常掌腱膜成纤维细胞之间的染色。由于Dupuytren病结节中独特的收缩细胞类型具有非肌肉性质，我们认为当将肌成纤维细胞这一术语应用于这种致病细胞类型时应被视为用词不当。
