Baker C J, Webb B J, Kasper D L, Edwards M S
J Infect Dis. 1986 Jul;154(1):47-54. doi: 10.1093/infdis/154.1.47.
The role of complement and antibody in the opsonophagocytosis of type II group B streptococci (type II GBS) was defined with sera from healthy adults and two populations with theoretical susceptibility to type II GBS infection--neonates and insulin-dependent diabetics. Significant opsonophagocytosis (bactericidal index, greater than or equal to 90%) of five clinical isolates of type II GBS lacking components of protein antigen c was demonstrated by each of 12 adult sera, as well as by agammaglobulinemic serum, a result indicating that opsonophagocytosis can proceed by antibody-independent activation of the classic complement pathway. Strains containing components of protein antigen c were somewhat more resistant to opsonin-binding activity. Four of 10 neonatal sera and nine of 15 diabetic sera exhibited inefficient opsonophagocytosis. Some of the adult sera with either high or low concentrations of specific antibody to type II GBS promoted opsonophagocytosis via the alternative complement pathway, but this response was not observed with neonatal sera. The addition of sufficient amounts of specific antibody to type II GBS to neonatal and adult diabetic sera in vitro, however, promoted efficient opsonophagocytosis via the alternative pathway.
通过健康成年人以及理论上易感染II型B组链球菌（II型GBS）的两类人群——新生儿和胰岛素依赖型糖尿病患者的血清，确定了补体和抗体在II型GBS调理吞噬作用中的作用。12份成人血清以及无丙种球蛋白血症血清均证明，对5株缺乏蛋白抗原c成分的II型GBS临床分离株具有显著的调理吞噬作用（杀菌指数大于或等于90%），这一结果表明，调理吞噬作用可通过经典补体途径的抗体非依赖性激活来进行。含有蛋白抗原c成分的菌株对调理素结合活性的抵抗力稍强。10份新生儿血清中的4份以及15份糖尿病患者血清中的9份表现出低效的调理吞噬作用。一些含有高浓度或低浓度II型GBS特异性抗体的成人血清通过替代补体途径促进调理吞噬作用，但在新生儿血清中未观察到这种反应（现象）。然而，在体外向新生儿和成年糖尿病患者血清中添加足量的II型GBS特异性抗体，可通过替代途径促进有效的调理吞噬作用。
