Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Theranostics. 2022 Jan 24;12(4):1829-1854. doi: 10.7150/thno.68804. eCollection 2022.
Natural active products (NAPs) are derived from chemical substances found in nature that have biological activity and medicinal potential. Screening and revealing the protein targets of NAPs is an indispensable link in the pharmacological and toxicological understanding of NAPs. Proteins are the main factors executing cell functions, and cells rely on the function of proteins to complete various activities in the life cycle. The important mechanism of action of drugs is to regulate cell biological activities by interacting with proteins and other macromolecules. At present, the classic way to screen protein targets is based on the molecular label tracing method, which has a long cycle and changes the molecular structure and pharmacological effects of NAPs. Due to the shortcomings of molecular labelling methods, in recent years, scientists have tried to develop a variety of label-free protein target identification methods for NAPs and have made a certain amount of progress. This article reviews the current protein target identification methods for NAPs with the aim of providing a reference for research on NAP protein targets.
天然活性产物（NAPs）是从自然界中存在的具有生物活性和药用潜力的化学物质中衍生而来的。筛选和揭示 NAPs 的蛋白靶标是理解 NAPs 药理学和毒理学的不可或缺的环节。蛋白质是执行细胞功能的主要因素，细胞依赖于蛋白质的功能来完成生命周期中的各种活动。药物的重要作用机制是通过与蛋白质和其他大分子相互作用来调节细胞的生物活性。目前，筛选蛋白靶标的经典方法是基于分子标记追踪法，但该方法周期长，会改变 NAPs 的分子结构和药理作用。由于分子标记方法的缺点，近年来，科学家们试图开发多种用于 NAPs 的无标记蛋白靶标鉴定方法，并取得了一定的进展。本文综述了 NAPs 的蛋白靶标鉴定方法，以期为 NAPs 蛋白靶标的研究提供参考。
