Rapid Detection and Differentiation of and Non Species by Using Recombinase Polymerase Amplification Combined With EuNPs-Based Lateral Flow Immunochromatography.

, a waterborne pathogen, is the main cause of Legionnaires' disease. Therefore, timely and accurate detection and differentiation of and non species is crucial. In this study, we develop an easy and rapid recombinase polymerase amplification assay combined with EuNPs-based lateral flow immunochromatography (EuNPs-LFIC-RPA) to specifically distinguish and non. We designed primers based on the gene of and the 5S rRNA gene of non- The recombinase polymerase amplification reaction could go to completion in 10 min at 37°C, and the amplification products could be detected within 5 min with EuNPs-LFIC strips. Using a florescent test strip reader, the quantitative results were achieved by reading the colored signal intensities on the strips. The sensitivity was 1.6 × 10 CFU/ml, and a linear standard linear curve plotted from the test strip reader had a correlation coefficient for the determination of ( = 0.9516). Completed concordance for the presence or absence of by EuNPs-LFIC-RPA and qPCR was 97.32% ( = 0.79, 95% CI), according to an analysis of practical water samples ( = 112). In short, this work shows the feasibility of EuNPs-LFIC-RPA for efficient and rapid monitoring of and non- in water samples.