Federal Food Safety and Veterinary Office, Berne, Switzerland.
Food Safety and Veterinary Office, Canton of Basel-Landschaft, Liestal, Switzerland.
J Appl Microbiol. 2018 Oct;125(4):1216-1225. doi: 10.1111/jam.13932. Epub 2018 Jul 23.
A molecular method for a rapid detection of viable Legionella pneumophila of all serogroups in tap water samples was developed as an alternative to the reference method (ISO). Legionellae are responsible for Legionnaires' disease, a severe pneumonia in humans with high lethality.
The developed method is based on a nutritional stimulation and detection of an increase in precursor 16S rRNA as an indicator for viability. For quantification, DNA was detected by qPCR. This method was compared to the ISO method using water samples obtained from public sports facilities in Switzerland. The sensitivity and specificity were 91 and 97%, respectively, when testing samples for compliance with a microbiological criterion of 1000 cell equivalents per l.
The new method is sensitive and specific for Leg. pneumophila and allows results to be obtained within 8 h upon arrival, compared to one week or more by the ISO method.
The method represents a useful tool for a rapid detection of viable Leg. pneumophila of all serogroups in water by molecular biology. It can be used as an alternative to the ISO method for official water analysis for legionellae and particularly when a short test time is required.
开发了一种分子方法，用于快速检测自来水中所有血清群的存活嗜肺军团菌，作为参考方法（ISO）的替代方法。军团菌可引起军团病，这是一种严重的人类肺炎，死亡率很高。
所开发的方法基于营养刺激和检测前 16S rRNA 的增加作为存活的指标。通过 qPCR 检测 DNA。使用从瑞士公共体育设施获得的水样将该方法与 ISO 方法进行比较。当测试样品是否符合每升 1000 个细胞当量的微生物标准时，该方法的灵敏度和特异性分别为 91%和 97%。
与 ISO 方法相比，新方法对嗜肺军团菌敏感且特异性高，可在到达后 8 小时内获得结果，而 ISO 方法则需要一周或更长时间。
该方法通过分子生物学为水中所有血清群的存活嗜肺军团菌的快速检测提供了一种有用的工具。它可以替代 ISO 方法用于军团菌的官方水质分析，特别是在需要短测试时间的情况下。
