通过电穿孔实现单细胞绿藻球 C-169 的稳定转化。
Stable transformation of unicellular green alga Coccomyxa subellipsoidea C-169 via electroporation.
机构信息
Department of Molecular Plant Physiology, University of Warsaw, Warsaw, Poland.
出版信息
Protoplasma. 2020 Mar;257(2):607-611. doi: 10.1007/s00709-019-01447-2. Epub 2019 Nov 18.
PMID:31741062
Abstract
In this study, we have shown the applicability of electroporation and hygromycin B as a convenient selectable marker for stable nuclear transformation of Coccomyxa subellipsoidea C-169. Since it is the first sequenced eukaryotic microorganism from polar environment, this offers unique opportunities to study adaptation mechanisms to cold.
摘要
在本研究中，我们展示了电穿孔和潮霉素 B 作为一种方便的选择标记，用于 Coccomyxa subellipsoidea C-169 的稳定核转化。由于它是第一个来自极地环境的测序真核微生物，这为研究适应寒冷的机制提供了独特的机会。
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