The Forsyth Institute, Cambridge, MA 02142, USA.
STAR Protoc. 2022 Feb 8;3(1):101167. doi: 10.1016/j.xpro.2022.101167. eCollection 2022 Mar 18.
This protocol outlines the process of preparing Saccharibacteria (TM7) and applying ligature with and without TM7 onto a mouse molar, and measuring the subsequent bone resorption and inflammation. This ligature model is particularly useful in studying the pathogenicity of specific bacteria that do not typically colonize the mouse oral cavity. This is especially true in the case of TM7 bacteria that prefer to grow on the surface of other bacteria. For complete details on the use and execution of this protocol, please refer to Chipashvili et al. (2021).
本方案概述了 Saccharibacteria（TM7）的制备过程，并在小鼠磨牙上应用带有和不带有 TM7 的结扎线，以测量随后的骨吸收和炎症情况。该结扎模型特别适用于研究特定细菌的致病性，这些细菌通常不会在小鼠口腔中定植。对于喜欢在其他细菌表面生长的 TM7 细菌来说尤其如此。如需详细了解本方案的使用和实施方法，请参阅 Chipashvili 等人（2021 年）。
