Song Peijia, Hao Yibing, Lin Degui, Jin Yipeng, Lin Jiahao
Country National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
China Veterinary Medicine Innovation Center, China Agricultural University, Beijing, China.
Front Microbiol. 2024 Jan 5;14:1329772. doi: 10.3389/fmicb.2023.1329772. eCollection 2023.
Periodontal disease (PD) is a prevalent oral affliction in canines, with limited therapeutic options available. The potential transmission of oral bacteria from canines to humans through inter-species contact poses a risk of zoonotic infection. Epigallocatechin gallate (EGCG), the principal catechin in green tea polyphenols, exhibits antibacterial properties effective against human PD. Given the clinical parallels between canine and human PD, this study explores the feasibility of employing EGCG as a therapeutic agent for canine PD.
Initially, a survey and statistical analysis of bacterial infection data related to canine PD in China were conducted. Subsequently, the primary pathogenic bacteria of canine PD were isolated and cultivated, and the antibacterial efficacy of EGCG was assessed. Furthermore, verify the therapeutic effect of EGCG on a mouse PD model . The high-throughput 16S rRNA gene sequencing identified and as the genera that distinguishing PD from healthy canines' gingival crevicular fluid (GCF) samples in China. The anaerobic culture and drug susceptibility testing isolated a total of 92 clinical strains, representing 22 species, from 72 canine GCF samples, including , , , etc. The minimum inhibitory concentration (MIC) ranging of EGCG was from 0.019 to 1.25 mg/mL. Following a 7 days oral mucosal administration of medium-dose EGCG (0.625 mg/mL), the abundance of periodontal microorganisms in PD mice significantly decreased. This intervention ameliorated alveolar bone loss, reducing the average cementoenamel junction to the alveolar bone crest (CEJ-ABC) distance from 0.306 mm ± 0.050 mm to 0.161 mm ± 0.026 mm. Additionally, EGCG (0.3125 mg/mL) markedly down-regulated the expression of inflammatory factor IL-6 in the serum of PD mice.
Our research demonstrates the significant antibacterial effects of EGCG against the prevalent bacterium in canine PD. Moreover, EGCG exhibits anti-inflammatory properties and proves effective in addressing bone loss in a PD mouse model. These findings collectively suggest the therapeutic potential of EGCG in the treatment of canine PD. The outcomes of this study contribute valuable data, laying the foundation for further exploration and screening of alternative antibiotic drugs to advance the management of canine PD.
牙周病（PD）是犬类中一种常见的口腔疾病，可用的治疗选择有限。口腔细菌通过种间接触从犬类传播给人类，存在人畜共患感染的风险。表没食子儿茶素没食子酸酯（EGCG）是绿茶多酚中的主要儿茶素，具有有效对抗人类牙周病的抗菌特性。鉴于犬类和人类牙周病在临床上的相似性，本研究探讨了将EGCG用作犬类牙周病治疗剂的可行性。
首先，对中国犬类牙周病相关细菌感染数据进行了调查和统计分析。随后，分离培养了犬类牙周病的主要病原菌，并评估了EGCG的抗菌效果。此外，验证了EGCG对小鼠牙周病模型的治疗效果。高通量16S rRNA基因测序确定了 和 为在中国区分牙周病犬与健康犬牙龈沟液（GCF）样本的属。厌氧培养和药敏试验从72份犬类GCF样本中分离出总共92株临床菌株，代表22个物种，包括 、 、 等。EGCG的最低抑菌浓度（MIC）范围为0.019至1.25mg/mL。在对牙周病小鼠进行7天的中剂量EGCG（0.625mg/mL）口腔黏膜给药后，可以发现牙周微生物的丰度显著降低。这种干预改善了牙槽骨吸收，将平均牙骨质釉质界至牙槽嵴顶（CEJ-ABC）距离从0.306mm±0.050mm减小至0.161mm±0.026mm。此外，EGCG（0.3125mg/mL）显著下调了牙周病小鼠血清中炎症因子IL-6的表达。
我们的研究证明了EGCG对犬类牙周病中常见细菌 具有显著的抗菌作用。此外，EGCG具有抗炎特性，并在牙周病小鼠模型中有效解决了骨质流失问题。这些发现共同表明EGCG在治疗犬类牙周病方面具有治疗潜力。本研究结果提供了有价值的数据，为进一步探索和筛选替代抗生素药物以推进犬类牙周病的管理奠定了基础。
