几丁质支架负载骨膜蛋白增强胸骨切开术后大鼠胸骨愈合，并降低血清 TNF-α 和 IL-6 水平。
Chitosan Scaffold Containing Periostin Enhances Sternum Bone Healing and Decreases Serum Level of TNF-α and IL-6 after Sternotomy in Rat.
机构信息
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
School of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, Iran.
出版信息
Tissue Eng Regen Med. 2022 Aug;19(4):839-852. doi: 10.1007/s13770-022-00434-8. Epub 2022 Feb 23.
BACKGROUND
In the aftermath of bone injuries, such as cranium and sternum, bone wax (BW) is used to control bleeding from the bone surfaces during surgery. Made up of artificial substances, however, it is associated with many complications such as inflammation, increased risk for infection, and bone repair delay. We, therefore, in this study set out to design and evaluate a novel BW without the above-mentioned side-effects reported for other therapies.
METHODS
The pastes (new BW(s)) were prepared in the laboratory and examined by MTT, MIC, MBC, and degradability tests. Then, 60 adult male Wistar rats, divided into six equal groups including chitosan (CT), CT-octacalcium phosphate (OCP), CT-periostin (Post), CT-OCP-Post, Control (Ctrl), and BW, underwent sternotomy surgery. Once the surgeries were completed, the bone repair was assessed radiologically and thereafter clinically in vivo and in vitro using CT-scan, H&E, ELISA, and qRT-PCR.
RESULTS
All pastes displayed antibacterial properties and the CT-Post group had the highest cell viability compared to the control group. In contrast to the BW, CT-Post group demonstrated weight changes in the degradability test. In the CT-Post group, more number of osteocyte cells, high trabeculae percentage, and the least fibrous connective tissue were observed compared to other groups. Additionally, in comparison to the CT and Ctrl groups, higher alkaline phosphatase activity, as well as decreased level of serum tumor necrosis factor-α, interleukin-6, and OCN in the CT-Post group was evident. Finally, Runx2, OPG, and RANKL genes' expression was significantly higher in the CT-Post group than in other groups.
CONCLUSION
Our results provide insights into the desirability of pastes in terms of cellular viability, degradability, antibacterial properties, and surgical site restoration compared to the BW group. Besides, Periostin could enhance the osteogenic properties of bone tissue defect site.
背景
在颅骨和胸骨等骨损伤后，骨蜡（BW）用于控制手术过程中骨表面的出血。然而，由于它是由人工物质组成的，因此与许多并发症相关，如炎症、感染风险增加和骨修复延迟。因此，在这项研究中，我们着手设计和评估一种新型 BW，避免其他治疗方法报告的上述副作用。
方法
在实验室中制备糊剂（新型 BW(s)），并通过 MTT、MIC、MBC 和降解性测试进行检查。然后，将 60 只成年雄性 Wistar 大鼠分为六组，包括壳聚糖（CT）、CT-八钙磷酸盐（OCP）、CT-骨膜蛋白（Post）、CT-OCP-Post、对照组（Ctrl）和 BW，进行胸骨切开术。一旦手术完成，通过 X 射线和体外 CT 扫描、H&E、ELISA 和 qRT-PCR 进行体内和体外评估骨修复情况。
结果
所有糊剂均显示出抗菌性能，与对照组相比，CT-Post 组的细胞活力最高。与 BW 组相比，CT-Post 组在降解性测试中显示出体重变化。在 CT-Post 组中，与其他组相比，观察到更多的成骨细胞数量、高骨小梁百分比和最少的纤维结缔组织。此外，与 CT 和 Ctrl 组相比，CT-Post 组碱性磷酸酶活性较高，血清肿瘤坏死因子-α、白细胞介素-6 和 OCN 水平较低。最后，CT-Post 组 Runx2、OPG 和 RANKL 基因的表达明显高于其他组。
结论
与 BW 组相比，我们的结果提供了关于糊剂在细胞活力、降解性、抗菌性能和手术部位恢复方面的优势的见解。此外，骨膜蛋白可以增强骨组织缺损部位的成骨特性。
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