Duchamp de Lageneste Oriane, Julien Anaïs, Abou-Khalil Rana, Frangi Giulia, Carvalho Caroline, Cagnard Nicolas, Cordier Corinne, Conway Simon J, Colnot Céline
INSERM UMR1163, Imagine Institute, Paris Descartes University, 75015, Paris, France.
Paris-Descartes Bioinformatics Platform, 75015, Paris, France.
Nat Commun. 2018 Feb 22;9(1):773. doi: 10.1038/s41467-018-03124-z.
Bone regeneration relies on the activation of skeletal stem cells (SSCs) that still remain poorly characterized. Here, we show that periosteum contains SSCs with high bone regenerative potential compared to bone marrow stromal cells/skeletal stem cells (BMSCs) in mice. Although periosteal cells (PCs) and BMSCs are derived from a common embryonic mesenchymal lineage, postnatally PCs exhibit greater clonogenicity, growth and differentiation capacity than BMSCs. During bone repair, PCs can efficiently contribute to cartilage and bone, and integrate long-term after transplantation. Molecular profiling uncovers genes encoding Periostin and other extracellular matrix molecules associated with the enhanced response to injury of PCs. Periostin gene deletion impairs PC functions and fracture consolidation. Periostin-deficient periosteum cannot reconstitute a pool of PCs after injury demonstrating the presence of SSCs within periosteum and the requirement of Periostin in maintaining this pool. Overall our results highlight the importance of analyzing periosteum and PCs to understand bone phenotypes.
骨再生依赖于仍未得到充分表征的骨骼干细胞（SSCs）的激活。在此，我们表明，与小鼠骨髓基质细胞/骨骼干细胞（BMSCs）相比，骨膜含有具有高骨再生潜力的SSCs。尽管骨膜细胞（PCs）和BMSCs源自共同的胚胎间充质谱系，但出生后PCs比BMSCs表现出更大的克隆形成能力、生长和分化能力。在骨修复过程中，PCs可以有效地促成软骨和骨的形成，并在移植后长期整合。分子图谱分析揭示了编码骨膜蛋白和其他与PCs对损伤增强反应相关的细胞外基质分子的基因。骨膜蛋白基因缺失会损害PCs的功能和骨折愈合。骨膜蛋白缺陷的骨膜在损伤后无法重建PCs库，这证明了骨膜内存在SSCs以及骨膜蛋白在维持该细胞库中的必要性。总体而言，我们的结果突出了分析骨膜和PCs以了解骨表型的重要性。
