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Visualization of the movement of the Escherichia coli RNA polymerase along the lac UV5 promoter during the initiation of the transcription.

作者信息

Spassky A

出版信息

J Mol Biol. 1986 Mar 5;188(1):99-103. doi: 10.1016/0022-2836(86)90484-5.

DOI:10.1016/0022-2836(86)90484-5
PMID:3519983
Abstract

Three probes have been used to detect changes in the contacts between Escherichia coli RNA polymerase and lac UV5 promoter during the formation of the open complex and the initiation of the transcription. The results presented here show how contacts between the enzyme and the UV5 promoter are modulated concomitantly with steady-state synthesis of ApApUpU. These results suggest a movement of the enzyme during the initiation of the transcription preceding the irreversible translocation into the elongation complex.

摘要

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Visualization of the movement of the Escherichia coli RNA polymerase along the lac UV5 promoter during the initiation of the transcription.
J Mol Biol. 1986 Mar 5;188(1):99-103. doi: 10.1016/0022-2836(86)90484-5.
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Effect of alkylating agents on initiation and elongation of the lac UV5 promoter.烷化剂对lac UV5启动子起始和延伸的影响。
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Cycling of ribonucleic acid polymerase to produce oligonucleotides during initiation in vitro at the lac UV5 promoter.在体外lac UV5启动子起始阶段，核糖核酸聚合酶循环以产生寡核苷酸。
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