Simpson R B
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3233-7. doi: 10.1073/pnas.76.7.3233.
I have identified those 5 positions of thymines in the lac UV5 promoter that lie close to bound Escherichia coli RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6). Although ultraviolet irradiation of DNA with 5-bromouracil substituted in place of thymine normally cleaves the DNA at the bromouracils, a protein bound to the DNA can perturb these cleavages at those locations at which the protein lies close to the bromine. In the lac promoter most of these contacts lie in three regions. Four contacts lie in the region where transcription initiates; four lie in the "Pribnow box," which is located about 10 base pairs upstream from the initiation site; and three more lie in the "-35 region," located about 35 base pairs upstream from the initiation site. The "Pribnow box" and the "-35 region" are regions whose sequences are partially conserved between promoters and in which most promoter mutations are located; thus, contacts in these two regions probably represent sites of sequence-specific recognition by RNA polymerase.
我已确定了乳糖UV5启动子中与结合的大肠杆菌RNA聚合酶（核苷三磷酸：RNA核苷酸转移酶，EC 2.7.7.6）接近的5个胸腺嘧啶位置。尽管用5-溴尿嘧啶取代胸腺嘧啶对DNA进行紫外线照射通常会在溴尿嘧啶处切割DNA，但与DNA结合的蛋白质会在蛋白质靠近溴的位置干扰这些切割。在乳糖启动子中，这些接触大多位于三个区域。四个接触位于转录起始区域；四个位于“Pribnow框”，其位于起始位点上游约10个碱基对处；另外三个位于“-35区域”，位于起始位点上游约35个碱基对处。“Pribnow框”和“-35区域”是启动子之间序列部分保守且大多数启动子突变位于其中的区域；因此，这两个区域中的接触可能代表RNA聚合酶进行序列特异性识别的位点。
