Department of Oncology, Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Bioengineered. 2022 Mar;13(3):5827-5842. doi: 10.1080/21655979.2022.2036406.
Circular RNAs (circRNAs) are associated with the progression of gastric cancer (GC). This study investigates the regulation of the circular RNA, hsa_circ_0119412 in GC and its effects on GC cells. The expression of hsa_circ_0119412, microRNA (, and zinc finger BED-type containing 3 (ZBED3) were measured by quantitative reverse transcription-PCR (qRT-PCR) and Western blotting. The cell counting kit-8 (CCK-8) assay, flow cytometry, transwell, and animal assays were performed to identify the roles of hsa_circ_0119412, , and ZBED3 in the viability, apoptosis, invasion, and growth of GC cells. The relationship between hsa_circ_0119412, , and was confirmed by luciferase, RNA immunoprecipitation (RIP), and RNA pull-down assays. Our data revealed that hsa_circ_0119412 and ZBED3 expression was upregulated in GC, while expression was downregulated. Both the knockdown of hsa_circ_0119412/ZBED3 and overexpression inhibited GC cell growth and invasion, and enhanced cell apoptosis, while interference or overexpression showed the opposite trend. Mechanistically, hsa_circ_0119412 sponges , which regulates ZBED3 expression. Silencing hsa_circ_0119412 inhibits the progression of GC, at least in part, by targeting the /ZBED3 axis.
环状 RNA（circRNAs）与胃癌（GC）的进展有关。本研究探讨了环状 RNA hsa_circ_0119412 在 GC 中的调控及其对 GC 细胞的影响。通过定量逆转录-聚合酶链反应（qRT-PCR）和 Western blot 测定 hsa_circ_0119412、微小 RNA（miRNA）和锌指 BED 型包含 3（ZBED3）的表达。通过细胞计数试剂盒-8（CCK-8）测定、流式细胞术、Transwell 和动物实验来鉴定 hsa_circ_0119412、和 ZBED3 在 GC 细胞活力、凋亡、侵袭和生长中的作用。通过荧光素酶、RNA 免疫沉淀（RIP）和 RNA 下拉实验证实了 hsa_circ_0119412、和之间的关系。我们的数据表明，hsa_circ_0119412 和 ZBED3 在 GC 中表达上调，而 miRNA 表达下调。hsa_circ_0119412/ ZBED3 敲低和 miRNA 过表达均抑制 GC 细胞生长和侵袭，增强细胞凋亡，而 miRNA 干扰或过表达则呈现相反趋势。机制上，hsa_circ_0119412 作为 miRNA 的海绵，调节 ZBED3 的表达。沉默 hsa_circ_0119412 通过靶向 /ZBED3 轴抑制 GC 的进展，至少在部分程度上是这样。
