Jeyaraman Madhan, Bingi Shiva Kumar, Muthu Sathish, Jeyaraman Naveen, Packkyarathinam Rathinavelpandian Perunchezhian, Ranjan Rajni, Sharma Shilpa, Jha Saurabh Kumar, Khanna Manish, Rajendran Sree Naga Sowndary, Rajendran Ramya Lakshmi, Gangadaran Prakash
Department of Orthopaedics, Faculty of Medicine, Sri Lalithambigai Medical College and Hospital, Dr MGR Educational and Research Institute, Chennai 600095, India.
Department of Biotechnology, School of Engineering and Technology, Sharda University, Greater Noida 201310, India.
Bioengineering (Basel). 2022 Jan 29;9(2):57. doi: 10.3390/bioengineering9020057.
Human bone marrow (BM) has been highlighted as a promising source of mesenchymal stromal cells (MSCs) containing various growth factors and cytokines that can be potentially utilized in regenerative procedures involving cartilage and bone. However, the proportion of MSCs in the nucleated cell population of BM is only around 0.001% to 0.01% thereby making the harvesting and processing technique crucial for obtaining optimal results upon its use in various regenerative processes. Although several studies in the literature have given encouraging results on the utility of BM aspiration concentrate (BMAC) in various regenerative procedures, there is a lack of consensus concerning the harvesting variables such as choice of anesthetic agent to be used, site of harvest, size of the syringe to be used, anticoagulant of choice, and processing variables such as centrifugation time, and speed. In this review article, we aim to discuss the variables in the harvesting and processing technique of BMAC and their impact on the yield of MSCs in the final concentrate obtained from them.
人骨髓（BM）已被视为间充质基质细胞（MSC）的一个有前景的来源，其含有多种生长因子和细胞因子，可潜在地用于涉及软骨和骨的再生程序。然而，骨髓有核细胞群中MSC的比例仅约为0.001%至0.01%，因此，收获和处理技术对于在各种再生过程中使用时获得最佳结果至关重要。尽管文献中的几项研究已就骨髓抽吸浓缩物（BMAC）在各种再生程序中的效用给出了令人鼓舞的结果，但在收获变量（如所用麻醉剂的选择、收获部位、所用注射器的尺寸、抗凝剂的选择）和处理变量（如离心时间和速度）方面仍缺乏共识。在这篇综述文章中，我们旨在讨论BMAC收获和处理技术中的变量及其对最终浓缩物中MSC产量的影响。
