Appl Opt. 2021 Dec 10;60(35):10825-10829. doi: 10.1364/AO.442065.
We present an external portable module for transforming bright-field microscopy to differential interference contrast (DIC) microscopy and digital holographic microscopy together. The module is composed of simple optical elements, positioned between the microscope output plane and the digital camera plane; thus, it can be integrated externally with existing microscopes. The proposed module enables polarization DIC imaging, without special polarization elements, under either white-light or coherent illumination, providing label-free imaging of biological samples, as recorded directly by the digital camera. In addition, by rotating one element inside the module, an off-axis hologram is created on the camera under coherent illumination, thus providing the possibility for reconstruction of the quantitative phase profile of the same sample. The method is demonstrated for imaging silica microspheres and biological cells.
我们提出了一种外部便携式模块，可将明场显微镜转换为微分干涉对比（DIC）显微镜和数字全息显微镜。该模块由简单的光学元件组成，位于显微镜输出平面和数字相机平面之间；因此，它可以与现有的显微镜外部集成。该模块可以在白光或相干照明下，通过旋转模块内的一个元件，在相机上创建离轴全息图，从而提供对生物样本的无标记成像，这些图像可直接由数字相机记录。无需特殊的偏振元件，即可实现偏振 DIC 成像，为同一样品的定量相位分布的重建提供了可能性。该方法已通过硅微球和生物细胞的成像得到了验证。
