使用离轴自干涉方法的定量 DIC 显微镜。
Quantitative DIC microscopy using an off-axis self-interference approach.
机构信息
G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
出版信息
Opt Lett. 2010 Jul 15;35(14):2370-2. doi: 10.1364/OL.35.002370.
Abstract
Traditional Normarski differential interference contrast (DIC) microscopy is a very powerful method for imaging nonstained biological samples. However, one of its major limitations is the nonquantitative nature of the imaging. To overcome this problem, we developed a quantitative DIC microscopy method based on off-axis sample self-interference. The digital holography algorithm is applied to obtain quantitative phase gradients in orthogonal directions, which leads to a quantitative phase image through a spiral integration of the phase gradients. This method is practically simple to implement on any standard microscope without stringent requirements on polarization optics. Optical sectioning can be obtained through enlarged illumination NA.
摘要
传统的诺马斯基微分干涉差（DIC）显微镜是一种非常强大的方法，用于对非染色生物样本进行成像。然而，它的一个主要限制是非成像的定量性质。为了克服这个问题，我们开发了一种基于离轴样品自干扰的定量 DIC 显微镜方法。数字全息算法被应用于获得正交方向的定量相位梯度，通过相位梯度的螺旋积分得到定量相位图像。该方法在任何标准显微镜上实现都非常简单，对偏光光学没有严格的要求。通过扩大照明 NA 可以获得光学切片。
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