Appl Opt. 2022 Feb 10;61(5):B77-B82. doi: 10.1364/AO.440376.
A digital lensless holographic microscope (DLHM) sensitive to the linear diattenuation produced by biological samples is reported. The insertion of a linear polarization-states generator and a linear polarization-states analyzer in a typical DLHM setup allows the proper linear diattenuation imaging of microscopic samples. The proposal has been validated for simulated and experimental biological samples containing calcium oxalate crystals extracted from agave leaves and potato starch grains. The performance of the proposed method is similar to that of a traditional polarimetric microscope to obtain linear diattenuation images of microscopic samples but with the advantages of DLHM, such as numerical refocusing, cost effectiveness, and the possibility of field-portable implementation.
报道了一种对生物样本产生的线性双折射敏感的数字无透镜全息显微镜 (DLHM)。在典型的 DLHM 设置中插入线性偏振态发生器和线性偏振态分析仪，可实现对微观样本的适当线性双折射成像。该方案已在包含从龙舌兰叶片中提取的草酸钙晶体和马铃薯淀粉颗粒的模拟和实验生物样本中得到验证。所提出的方法的性能与传统的偏振显微镜相似，可以获得微观样本的线性双折射图像，但具有 DLHM 的优势，如数值重聚焦、成本效益以及实现现场便携的可能性。
