在细胞模型中，错配结合配体上调了反式剪接反应，产生了环状 RNA。

Mismatch binding ligand upregulated back-splicing reaction producing circular RNA in a cellular model.

机构信息

SANKEN (The Institute of Scientific and Industrial Research), Osaka University, 8-1 Mihogaoka, Ibaraki 567-0047, Japan.

出版信息

Chem Commun (Camb). 2022 Mar 15;58(22):3629-3632. doi: 10.1039/d1cc06936e.

PMID:35201254

Abstract

Circular RNA (circRNA) is a covalently closed single-stranded RNA produced from pre-mRNAs back-splicing reaction, an alternative form of splicing. Here, we show naphthyridine carbamate dimer (NCD) upregulating the production of a circRNA from a pre-mRNA containing NCD-binding site UGGAA/UGGAA in cells, demonstrating the feasibility of small-molecule mediated circRNA production.

摘要

环状 RNA (circRNA) 是一种由前体 mRNA 反向剪接反应产生的共价闭合的单链 RNA，是一种选择性剪接的形式。在这里，我们展示了萘啶基氨基甲酸酯二聚体（NCD）在细胞中上调含有 NCD 结合位点 UGGAA/UGGAA 的前体 mRNA 产生 circRNA 的能力，证明了小分子介导 circRNA 产生的可行性。