Oh Yu-Ri, Jang Young-Ah, Song Jae Kwang, Eom Gyeong Tae
Bio-based Chemistry Research Center, Korea Research Institute of Chemical Technology (KRICT), Ulsan, 44429, Republic of Korea.
Bio-based Chemistry Research Center, Korea Research Institute of Chemical Technology (KRICT), Daejeon, 34114, Republic of Korea.
Bioprocess Biosyst Eng. 2022 May;45(5):901-909. doi: 10.1007/s00449-022-02708-w. Epub 2022 Feb 24.
Maltobionic acid (MBA) can be applied to various fields such as food, cosmetics, and pharmaceutical industries. In this study, whole-cell biocatalysis for MBA production was performed using recombinant Pseudomonas taetrolens homologously expressing quinoprotein glucose dehydrogenase (GDH). Various reaction parameters such as temperature, cell density, and cell harvest time, were optimized for improving MBA production. Under the optimized reaction conditions using pure maltose as a substrate, the MBA production titer, yield, and productivity of whole-cell biocatalyst (WCB) were 200 g/L, 95.6%, and 18.18 g/L/h, respectively, which were the highest compared to those reported previously. Productivity, a key factor for industrial MBA production, obtained from whole-cell biocatalysis in this study, was enhanced by approximately 1.9-fold compared to that obtained in our previous work (9.52 g/L/h) using the fermentation method. Additionally, the WCB could be reused up to six times without a significant reduction in MBA productivity, indicating that the WCB is very robust. Although MBA productivity (8.33 g/L/h) obtained from high-maltose corn syrup (HMCS) as a substrate was 45.8% of that using pure maltose, HMCS can be a better substrate for commercial MBA production because its price is only 1.1% of that of pure maltose. The results of this study using a WCB to convert maltose into MBA may support the development of a potential industrial process for more economically effective MBA production in the future.
麦芽仿生酸（MBA）可应用于食品、化妆品和制药等多个领域。在本研究中，使用同源表达醌蛋白葡萄糖脱氢酶（GDH）的重组类产碱假单胞菌进行了用于生产MBA的全细胞生物催化。对温度、细胞密度和细胞收获时间等各种反应参数进行了优化，以提高MBA的产量。在以纯麦芽糖为底物的优化反应条件下，全细胞生物催化剂（WCB）的MBA产量、产率和生产力分别为200 g/L、95.6%和18.18 g/L/h，与之前报道的相比是最高的。本研究中通过全细胞生物催化获得的生产力是工业生产MBA的关键因素，与我们之前使用发酵法的工作（9.52 g/L/h）相比提高了约1.9倍。此外，WCB可以重复使用多达六次，而MBA生产力没有显著降低，这表明WCB非常稳定。虽然以高麦芽糖玉米糖浆（HMCS）为底物获得的MBA生产力（8.33 g/L/h）是使用纯麦芽糖时的45.8%，但HMCS可以成为商业生产MBA的更好底物，因为其价格仅为纯麦芽糖的1.1%。本研究使用WCB将麦芽糖转化为MBA的结果可能支持未来开发一种更经济有效的MBA生产潜在工业工艺。
