长链非编码RNA POU3F3通过下调长链非编码RNA MEG3促进黑色素瘤细胞增殖。
LncRNA POU3F3 promotes melanoma cell proliferation by downregulating lncRNA MEG3.
作者信息
Liu Yingnan, Zhuang Yongqing, Fu Xiaokuan, Li Chaofei
机构信息
Department of Hand and Microvascular Surgery, Shenzhen People's Hospital, The Second Clinical Medical College, Jinan University, Shenzhen, 518000, Guangdong, China.
Department of Hand and Microvascular Surgery, Shenzhen People's Hospital, The First Affiliated Hospital,Southern University of Science and Technology, Shenzhen, 518000, Guangdong, China.
出版信息
Discov Oncol. 2021 Jul 9;12(1):21. doi: 10.1007/s12672-021-00414-9.
BACKGROUND
LncRNA POU3F3 (POU3F3) is overexpressed and plays oncogenic roles in esophageal squamous-cell carcinomas. LncRNA MEG3 (MEG3) has been characterized as a tumor suppressive lncRNA in different types of cancer. Our preliminary deep sequencing analysis revealed the inverse correlation between POU3F3 and MEG2 across melanoma tissues, indicating the interaction between them in melanoma. Therefore, this study was performed to investigate the crosstalk between POU3F3 and MEG3 in melanoma.
METHODS
Tumor and adjacent healthy tissues collected from 60 melanoma patients were subjected to RNA extractions and RT-qPCRs to analyze the differential expression of POU3F3 and MEG2 in melanoma. In melanoma cells, POU3F3 and MEG2 were overexpressed to study the interactions between them. CCK-8 assays were performed to analyze the roles of POU3F3 and MEG2 in regulating melanoma cell proliferation.
RESULTS
We found that POU3F3 was upregulated, while lncRNA MEG3 was downregulated in melanoma. Expression levels of POU3F3 and MEG3 were inversely correlated across tumor tissues. In vitro experiments showed that POU3F3 overexpression decreased MEG3 expression in melanoma cells, while MEG3 overexpression failed to affect POU3F3. POU3F3 overexpression increased melanoma cell proliferation, while MEG3 overexpression decreased melanoma cell proliferation. In addition, rescue experiments showed that MEG3 overexpression attenuated the enhancing effects of POU3F3 overexpression.
CONCLUSION
POU3F3 may promote melanoma cell proliferation by downregulating MEG3.
背景
长链非编码RNA POU3F3（POU3F3）在食管鳞状细胞癌中过表达并发挥致癌作用。长链非编码RNA MEG3（MEG3）在不同类型癌症中已被鉴定为肿瘤抑制性长链非编码RNA。我们初步的深度测序分析揭示了黑色素瘤组织中POU3F3和MEG2之间呈负相关，表明它们在黑色素瘤中存在相互作用。因此，本研究旨在探讨POU3F3和MEG3在黑色素瘤中的相互作用。
方法
收集60例黑色素瘤患者的肿瘤组织和相邻健康组织，进行RNA提取和逆转录定量聚合酶链反应（RT-qPCR），以分析POU3F3和MEG2在黑色素瘤中的差异表达。在黑色素瘤细胞中，过表达POU3F3和MEG2以研究它们之间的相互作用。进行细胞计数试剂盒-8（CCK-8）分析，以研究POU3F3和MEG2在调节黑色素瘤细胞增殖中的作用。
结果
我们发现黑色素瘤中POU3F3上调，而长链非编码RNA MEG3下调。肿瘤组织中POU3F3和MEG3的表达水平呈负相关。体外实验表明，POU3F3过表达降低了黑色素瘤细胞中MEG3的表达，而MEG3过表达未能影响POU3F3。POU3F3过表达增加了黑色素瘤细胞的增殖，而MEG3过表达降低了黑色素瘤细胞的增殖。此外，挽救实验表明，MEG3过表达减弱了POU3F3过表达的增强作用。
结论
POU3F3可能通过下调MEG3促进黑色素瘤细胞增殖。
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