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快速、多分析物检测废水中的阿片类代谢物。

Rapid, Multianalyte Detection of Opioid Metabolites in Wastewater.

机构信息

Department of Physics, Boston College, Chestnut Hill, Massachusetts 02467, United States.

Giner Inc., Newton, Massachusetts 02466, United States.

出版信息

ACS Nano. 2022 Mar 22;16(3):3704-3714. doi: 10.1021/acsnano.1c07094. Epub 2022 Feb 24.

DOI:10.1021/acsnano.1c07094
PMID:35201755
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9949512/
Abstract

By monitoring opioid metabolites, wastewater-based epidemiology (WBE) could be an excellent tool for real-time information on the consumption of illicit drugs. A key limitation of WBE is the reliance on costly laboratory-based techniques that require substantial infrastructure and trained personnel, resulting in long turnaround times. Here, we present an aptamer-based graphene field effect transistor (AptG-FET) platform for simultaneous detection of three different opioid metabolites. This platform provides a reliable, rapid, and inexpensive method for quantitative analysis of opioid metabolites in wastewater. The platform delivers a limit of detection 2-3 orders of magnitude lower than previous reports, but in line with the concentration range (pg/mL to ng/mL) of these opioid metabolites present in real samples. To enable multianalyte detection, we developed a facile, reproducible, and high-yield fabrication process producing 20 G-FETs with integrated side gate platinum (Pt) electrodes on a single chip. Our devices achieved the selective multianalyte detection of three different metabolites: noroxycodone (NX), 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), and norfentanyl (NF) in wastewater diluted 20× in buffer.

摘要

通过监测阿片类药物代谢物，基于污水的流行病学（WBE）可能成为实时了解非法药物消费情况的绝佳工具。WBE 的一个主要限制是依赖昂贵的基于实验室的技术，这些技术需要大量的基础设施和训练有素的人员，导致周转时间长。在这里，我们提出了一种基于适体的石墨烯场效应晶体管（AptG-FET）平台，用于同时检测三种不同的阿片类药物代谢物。该平台为污水中阿片类药物代谢物的定量分析提供了一种可靠、快速且廉价的方法。该平台的检测限比以前的报告低 2-3 个数量级，但与实际样品中存在的这些阿片类药物代谢物的浓度范围（pg/mL 到 ng/mL）一致。为了实现多分析物检测，我们开发了一种简便、可重复且高产的制造工艺，可在单个芯片上生产 20 个带有集成侧门铂（Pt）电极的 G-FET。我们的器件实现了对三种不同代谢物（羟考酮（NX）、2-亚乙基-1,5-二甲基-3,3-二苯基吡咯烷（EDDP）和去甲芬太尼（NF））的选择性多分析物检测，污水在缓冲液中稀释 20 倍。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6157/9949512/6f8137f543f7/nihms-1867956-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6157/9949512/46fe4cd07b66/nihms-1867956-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6157/9949512/319a00ca4874/nihms-1867956-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6157/9949512/12fb3e772526/nihms-1867956-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6157/9949512/461293c379cb/nihms-1867956-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6157/9949512/6f8137f543f7/nihms-1867956-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6157/9949512/46fe4cd07b66/nihms-1867956-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6157/9949512/319a00ca4874/nihms-1867956-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6157/9949512/12fb3e772526/nihms-1867956-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6157/9949512/461293c379cb/nihms-1867956-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6157/9949512/6f8137f543f7/nihms-1867956-f0006.jpg

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