Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States.
Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United States.
ACS Appl Mater Interfaces. 2023 Aug 9;15(31):37784-37793. doi: 10.1021/acsami.3c05958. Epub 2023 Jul 31.
The opioid crisis is a worldwide public health crisis that has affected millions of people. In recent years, synthetic opioids, primarily illicit fentanyl, have become the primary driver of overdose deaths. There is a great need for a highly sensitive, portable, and inexpensive analytical tool that can quickly indicate the presence and relative threat of fentanyl. In this work, we develop a semiconductor enriched (sc-) single-walled carbon nanotube (SWCNT)-based field-effect transistor (FET) biosensor functionalized with norfentanyl antibodies for the sensitive detection of norfentanyl, the primary inactive metabolite of fentanyl, in urine samples. Different sensor configurations were explored in order to obtain the most optimized sensing results. Moreover, by employing the "reduced" antibody, we achieved orientated immobilization of the norfentanyl antibody and thus brought the antigen-antibody interaction closer to the sensor surface, further improving the sensitivity. The reported norfentanyl biosensors have a limit of detection in the fg/mL region in both calibration samples and synthetic urine samples, showing ultrasensitivity and high reliability.
阿片类药物危机是一个全球性的公共卫生危机，影响了数百万人。近年来，合成阿片类药物，主要是非法芬太尼，已成为导致过量死亡的主要因素。因此，我们非常需要一种高灵敏度、便携式和廉价的分析工具，能够快速指示芬太尼的存在和相对威胁。在这项工作中，我们开发了一种基于半导体富集（sc）单壁碳纳米管（SWCNT）的场效应晶体管（FET）生物传感器，该传感器用纳洛啡抗体进行功能化，用于灵敏检测尿液样品中的纳洛啡，纳洛啡是芬太尼的主要无活性代谢物。我们探索了不同的传感器配置，以获得最优化的传感结果。此外，通过使用“还原”抗体，我们实现了纳洛啡抗体的定向固定，从而使抗原-抗体相互作用更接近传感器表面，进一步提高了灵敏度。所报道的纳洛啡生物传感器在校准样品和合成尿液样品中的检测限均在 fg/mL 区域内，表现出超灵敏性和高可靠性。
