IEEE Trans Nanobioscience. 2022 Jul;21(3):387-394. doi: 10.1109/TNB.2022.3154438. Epub 2022 Jun 30.
Gold Nanoparticles (GNPs) have shown promising capabilities for use in many in-vivo applications such as gene and drug delivery, photothermal ablation of tumors, and tracking in many imaging modalities. Yet GNPs have thus far had limited use in cardiovascular medicine. Polyethylene glycol functionalized (PEGylated) GNPs have been extensively studied in a wide array of in vitro and in vivo models with results showing no apparent toxicity, but to our knowledge an investigation has never been performed to determine direct cardiomyocyte toxicity. In this study, we assessed if PEGylated GNPs exhibited direct toxicity to a primary culture of neonatal rat cardiomyocytes in order to establish PEGylated GNPs for potential future use in cardiovascular medicine applications. We present novel results that demonstrate both a particle size and concentration dependent relationship on cell viability. Cell viability was found to be significantly enhanced for many concentrations and sizes as compared to the control and increased linearly as a function of particle diameter. Additionally, viability increased in a parabolically dependent manner as a function of decreasing particle concentration. These new results could advance understanding of nanoparticle-cell interactions and lead to the development of new applications involving the use of gold nanoparticles in cardiovascular medicine.
金纳米粒子（GNPs）在许多体内应用中显示出有前途的应用潜力，例如基因和药物传递、肿瘤光热消融以及在多种成像方式中的跟踪。然而，GNPs 在心血管医学中的应用迄今为止受到限制。聚乙二醇功能化（PEGylated）GNPs 在广泛的体外和体内模型中进行了广泛的研究，结果表明没有明显的毒性，但据我们所知，从未进行过研究以确定直接心肌细胞毒性。在这项研究中，我们评估了 PEGylated GNPs 是否对原代培养的新生大鼠心肌细胞表现出直接毒性，以便为心血管医学应用中潜在的未来使用建立 PEGylated GNPs。我们提出了新的结果，证明了细胞活力与颗粒大小和浓度的依赖性关系。与对照组相比，许多浓度和尺寸的细胞活力明显增强，并随粒径呈线性增加。此外，随着粒径的降低，活力呈抛物线依赖性增加。这些新结果可以加深对纳米颗粒-细胞相互作用的理解，并导致涉及在心血管医学中使用金纳米粒子的新应用的发展。
