Pekkoh Jeeraporn, Phinyo Kittiya, Thurakit Theera, Lomakool Sureeporn, Duangjan Kritsana, Ruangrit Khomsan, Pumas Chayakorn, Jiranusornkul Supat, Yooin Wipawadee, Cheirsilp Benjamas, Pathom-Aree Wasu, Srinuanpan Sirasit
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand.
Antioxidants (Basel). 2022 Jan 19;11(2):186. doi: 10.3390/antiox11020186.
Diatoms, as single cell eukaryotic microalgae, are rich sources of lipids, which have either beneficial or detrimental effects on the prevention and treatment of many diseases. Gas chromatography-mass spectrometry (GC-MS) identified diatom lipids with high levels of essential fatty acids (EFAs), especially polyunsaturated FAs (PUFAs) containing both omega-3 and omega-6. Nutritional values of FAs indicated possible applications in the pharmaceutical, nutraceutical, and functional food industries. Diatom FAs showed antioxidative potential on harmful radicals by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging, with high inhibition of the angiotensin-converting enzyme (ACE) that causes cardiovascular disease (CVD) and hypertension. A computational molecular docking simulation confirmed the inhibition mechanisms of FAs on ACE, with comparable levels of binding free energy to chemically synthesized ACE drugs. Findings suggested that diatom lipids showed potential for use as alternative ACE inhibitors or food supplement for CVD prevention.
硅藻作为单细胞真核微藻，是丰富的脂质来源，这些脂质对许多疾病的预防和治疗具有有益或有害的影响。气相色谱 - 质谱联用仪（GC-MS）鉴定出硅藻脂质含有高水平的必需脂肪酸（EFA），特别是同时含有ω-3和ω-6的多不饱和脂肪酸（PUFA）。脂肪酸的营养价值表明其在制药、营养保健品和功能性食品行业可能具有应用价值。硅藻脂肪酸通过2,2-二苯基-1-苦基肼（DPPH）和2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）清除对有害自由基表现出抗氧化潜力，对导致心血管疾病（CVD）和高血压的血管紧张素转换酶（ACE）具有高度抑制作用。计算分子对接模拟证实了脂肪酸对ACE的抑制机制，其结合自由能水平与化学合成的ACE药物相当。研究结果表明，硅藻脂质有潜力用作替代ACE抑制剂或用于预防CVD的食品补充剂。
