Rincón-Cervera Miguel Ángel, González-Barriga Valeria, Romero Jaime, Rojas Rodrigo, López-Arana Sandra
Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Avda. El Líbano 5524, Macul, Santiago 7830490, Chile.
Departamento de Acuicultura, Facultad de Ciencias del Mar, Universidad Católica del Norte, Larrondo 1281, Coquimbo 1781421, Chile.
Foods. 2020 Feb 21;9(2):233. doi: 10.3390/foods9020233.
Fatty acid composition and distribution in edible species of fish and shellfish captured in the South Pacific were studied, with a focus on n-3 long-chain polyunsaturated fatty acids (n-3 LCPUFA). Fatty acids were quantified using gas-chromatography coupled with flame ionization detection (GC-FID), and the distribution of different fatty acids within lipid classes (neutral and polar lipids) was achieved after oil extraction using the Folch method and separation of lipid classes via solid-phase extraction for further GC-FID analysis. Red cusk-eel was the fish species with the lowest content of both EPA and DHA (40.8 and 74.4 mg/100 g, respectively) whereas mackerel contained the highest amount (414.7 and 956.0 mg/100 g for EPA and DHA, respectively). Sea squirt was the shellfish species with the highest content of EPA and DHA (375.0 and 165.7 mg/100 g, respectively) whereas the lowest amount of EPA + DHA was found in Chilean abalone (63.6 mg/100 g). PUFA were mostly found in neutral or polar lipids depending on the studied species. Indexes used to discuss the nutritional quality of lipids (PUFA/SFA, n-6/n-3 ratio and the hypocholesterolemic/hypercholesterolemic fatty acid index) were calculated and reported in the manuscript. This information provides a novel nutritional insight which may be useful to help nutritionists and other health professionals give more accurate counseling for the population to reach the recommended daily intakes of EPA and DHA.
对在南太平洋捕获的食用鱼类和贝类的脂肪酸组成及分布进行了研究，重点关注n-3长链多不饱和脂肪酸（n-3 LCPUFA）。使用气相色谱-火焰离子化检测联用技术（GC-FID）对脂肪酸进行定量分析，采用Folch法提取油脂后，通过固相萃取分离脂质类别，以实现不同脂肪酸在脂质类别（中性脂质和极性脂质）中的分布，进而进行GC-FID分析。红盲鳗是二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）含量最低的鱼类（分别为40.8和74.4毫克/100克），而鲭鱼的含量最高（EPA和DHA分别为414.7和956.0毫克/100克）。海鞘是EPA和DHA含量最高的贝类（分别为375.0和165.7毫克/100克），而智利鲍鱼中EPA + DHA的含量最低（63.6毫克/100克）。根据所研究的物种不同，多不饱和脂肪酸（PUFA）大多存在于中性或极性脂质中。本文计算并报告了用于讨论脂质营养质量的指标（PUFA/SFA、n-6/n-3比值和降胆固醇/升胆固醇脂肪酸指数）。这些信息提供了新的营养见解，可能有助于营养学家和其他健康专业人员为民众提供更准确的建议，以达到EPA和DHA的每日推荐摄入量。
