来自大肠杆菌的标签基因的核苷酸序列。
Nucleotide sequence of the tag gene from Escherichia coli.
作者信息
Steinum A L, Seeberg E
出版信息
Nucleic Acids Res. 1986 May 12;14(9):3763-72. doi: 10.1093/nar/14.9.3763.
Abstract
We have determined the complete nucleotide sequence of the tag gene, encoding 3-methyladenine DNA glycosylase I from Escherichia coli. From the nucleotide sequence it is deduced that the tag enzyme consists of 187 amino-acids and has a calculated molecular weight of 21.1 kdaltons. The tag enzyme is unusually rich in cysteine (8 residues) with a cluster of three consecutive cysteines near the C-terminal end. The tag coded DNA glycosylase does not show significant sequence homology to the alkA coded glycosylase in spite of that both of these enzymes catalyze the release of free 3-methyladenine from alkylated DNA.
摘要
我们已经确定了编码来自大肠杆菌的3-甲基腺嘌呤DNA糖基化酶I的tag基因的完整核苷酸序列。从核苷酸序列推断，tag酶由187个氨基酸组成，计算分子量为21.1千道尔顿。tag酶的半胱氨酸含量异常丰富（8个残基），在C末端附近有三个连续半胱氨酸组成的簇。尽管tag编码的DNA糖基化酶和alkA编码的糖基化酶都催化从烷基化DNA中释放游离的3-甲基腺嘌呤，但它们之间没有显著的序列同源性。
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