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1
Nucleotide sequence of the tag gene from Escherichia coli.来自大肠杆菌的标签基因的核苷酸序列。
Nucleic Acids Res. 1986 May 12;14(9):3763-72. doi: 10.1093/nar/14.9.3763.
2
Amplified expression of the tag+ and alkA+ genes in Escherichia coli: identification of gene products and effects on alkylation resistance.大肠杆菌中tag+和alkA+基因的扩增表达：基因产物的鉴定及其对烷基化抗性的影响。
J Bacteriol. 1986 Nov;168(2):642-7. doi: 10.1128/jb.168.2.642-647.1986.
3
Cloning and characterization of a 3-methyladenine DNA glycosylase cDNA from human cells whose gene maps to chromosome 16.从人细胞中克隆并鉴定一种3-甲基腺嘌呤DNA糖基化酶cDNA，其基因定位于16号染色体。
Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9127-31. doi: 10.1073/pnas.88.20.9127.
4
Cloning and expression in Escherichia coli of a gene for an alkylbase DNA glycosylase from Saccharomyces cerevisiae; a homologue to the bacterial alkA gene.酿酒酵母中一种烷基碱基DNA糖基化酶基因的克隆及其在大肠杆菌中的表达；该基因是细菌alkA基因的同源物。
EMBO J. 1990 Dec;9(13):4563-8. doi: 10.1002/j.1460-2075.1990.tb07909.x.
5
Cloning of Escherichia coli genes encoding 3-methyladenine DNA glycosylases I and II.编码3-甲基腺嘌呤DNA糖基化酶I和II的大肠杆菌基因的克隆
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6
Purification and structure of 3-methyladenine-DNA glycosylase I of Escherichia coli.
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Excision of hypoxanthine from DNA containing dIMP residues by the Escherichia coli, yeast, rat, and human alkylpurine DNA glycosylases.大肠杆菌、酵母、大鼠和人类烷基嘌呤DNA糖基化酶从含有dIMP残基的DNA中切除次黄嘌呤。
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Purification and characterization of 3-methyladenine DNA glycosylase I from Escherichia coli.大肠杆菌3-甲基腺嘌呤DNA糖基化酶I的纯化与特性分析
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Bacillus subtilis alkA gene encoding inducible 3-methyladenine DNA glycosylase is adjacent to the ada operon.编码诱导型3-甲基腺嘌呤DNA糖基化酶的枯草芽孢杆菌alkA基因与ada操纵子相邻。
J Bacteriol. 1993 Sep;175(18):6010-7. doi: 10.1128/jb.175.18.6010-6017.1993.

引用本文的文献

1
Linkage map of Escherichia coli K-12, edition 10: the traditional map.大肠杆菌K-12连锁图谱，第10版：传统图谱。
Microbiol Mol Biol Rev. 1998 Sep;62(3):814-984. doi: 10.1128/MMBR.62.3.814-984.1998.
2
DNA glycosylases in the base excision repair of DNA.DNA碱基切除修复中的DNA糖基化酶。
Biochem J. 1997 Jul 1;325 ( Pt 1)(Pt 1):1-16. doi: 10.1042/bj3250001.
3
Excision of 3-methylguanine from alkylated DNA by 3-methyladenine DNA glycosylase I of Escherichia coli.大肠杆菌的3-甲基腺嘌呤DNA糖基化酶I从烷基化DNA中切除3-甲基鸟嘌呤。
Nucleic Acids Res. 1993 May 11;21(9):2045-9. doi: 10.1093/nar/21.9.2045.
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Physical and genetic mapping of the tag gene on the Escherichia coli chromosome.大肠杆菌染色体上标签基因的物理图谱和遗传图谱
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Increased removal of 3-alkyladenine reduces the frequencies of hprt mutations induced by methyl- and ethylmethanesulfonate in Chinese hamster fibroblast cells.增加 3-烷基腺嘌呤的去除可降低甲基磺酸甲酯和甲基磺酸乙酯在中国仓鼠成纤维细胞中诱导的 hprt 突变频率。
Nucleic Acids Res. 1994 May 11;22(9):1670-4. doi: 10.1093/nar/22.9.1670.
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DNA glycosylase enzymes induced during chemical adaptation of M. luteus.藤黄微球菌化学适应性诱导过程中产生的DNA糖基化酶
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7
Purification and characterization of 3-methyladenine DNA glycosylase I from Escherichia coli.大肠杆菌3-甲基腺嘌呤DNA糖基化酶I的纯化与特性分析
Nucleic Acids Res. 1987 Apr 10;15(7):2787-801. doi: 10.1093/nar/15.7.2787.
8
Amplified expression of the tag+ and alkA+ genes in Escherichia coli: identification of gene products and effects on alkylation resistance.大肠杆菌中tag+和alkA+基因的扩增表达：基因产物的鉴定及其对烷基化抗性的影响。
J Bacteriol. 1986 Nov;168(2):642-7. doi: 10.1128/jb.168.2.642-647.1986.
9
Formamidopyrimidine-DNA glycosylase of Escherichia coli: cloning and sequencing of the fpg structural gene and overproduction of the protein.大肠杆菌的甲酰胺嘧啶-DNA糖基化酶：fpg结构基因的克隆与测序及该蛋白质的过量表达
EMBO J. 1987 Oct;6(10):3177-83. doi: 10.1002/j.1460-2075.1987.tb02629.x.
10
A second DNA methyltransferase repair enzyme in Escherichia coli.
Proc Natl Acad Sci U S A. 1988 May;85(9):3039-43. doi: 10.1073/pnas.85.9.3039.

本文引用的文献

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A comprehensive quantitative analysis of methylated and ethylated DNA using high pressure liquid chromatography.使用高压液相色谱法对甲基化和乙基化DNA进行全面的定量分析。
Carcinogenesis. 1980 Jul;1(7):595-606. doi: 10.1093/carcin/1.7.595.
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A simple method for displaying the hydropathic character of a protein.一种展示蛋白质亲水性特征的简单方法。
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Two DNA glycosylases in Escherichia coli which release primarily 3-methyladenine.大肠杆菌中的两种DNA糖基化酶，主要释放3-甲基腺嘌呤。
Biochemistry. 1982 Mar 16;21(6):1162-9. doi: 10.1021/bi00535a009.
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Adaptation to alkylation resistance involves the induction of a DNA glycosylase.对烷化抗性的适应涉及一种DNA糖基化酶的诱导。
Nature. 1982 Apr 22;296(5859):773-5. doi: 10.1038/296773a0.
5
Induction of a DNA glycosylase for N-methylated purines is part of the adaptive response to alkylating agents.诱导一种针对N-甲基化嘌呤的DNA糖基化酶是对烷化剂适应性反应的一部分。
Nature. 1982 Apr 22;296(5859):770-3. doi: 10.1038/296770a0.
6
Escherichia coli mutants deficient in 3-methyladenine-DNA glycosylase.缺乏3-甲基腺嘌呤-DNA糖基化酶的大肠杆菌突变体。
J Mol Biol. 1980 Jun 15;140(1):101-27. doi: 10.1016/0022-2836(80)90358-7.
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Sequences of the recA gene and protein.recA基因和蛋白质的序列。
Proc Natl Acad Sci U S A. 1980 May;77(5):2611-5. doi: 10.1073/pnas.77.5.2611.
8
Cloning and characterization of the alkA gene of Escherichia coli that encodes 3-methyladenine DNA glycosylase II.编码3-甲基腺嘌呤DNA糖基化酶II的大肠杆菌alkA基因的克隆与特性分析。
J Biol Chem. 1984 Nov 25;259(22):13723-9.
9
The nucleotide sequence of the uvrD gene of E. coli.大肠杆菌uvrD基因的核苷酸序列。
Nucleic Acids Res. 1984 Jul 25;12(14):5789-99. doi: 10.1093/nar/12.14.5789.
10
Evidence for use of rare codons in the dnaG gene and other regulatory genes of Escherichia coli.大肠杆菌dnaG基因及其他调控基因中稀有密码子使用的证据。
Proc Natl Acad Sci U S A. 1983 Feb;80(3):687-91. doi: 10.1073/pnas.80.3.687.

来自大肠杆菌的标签基因的核苷酸序列。

Nucleotide sequence of the tag gene from Escherichia coli.

作者信息

Steinum A L, Seeberg E

出版信息

Nucleic Acids Res. 1986 May 12;14(9):3763-72. doi: 10.1093/nar/14.9.3763.

DOI:10.1093/nar/14.9.3763
PMID:3520491
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC339813/
Abstract

We have determined the complete nucleotide sequence of the tag gene, encoding 3-methyladenine DNA glycosylase I from Escherichia coli. From the nucleotide sequence it is deduced that the tag enzyme consists of 187 amino-acids and has a calculated molecular weight of 21.1 kdaltons. The tag enzyme is unusually rich in cysteine (8 residues) with a cluster of three consecutive cysteines near the C-terminal end. The tag coded DNA glycosylase does not show significant sequence homology to the alkA coded glycosylase in spite of that both of these enzymes catalyze the release of free 3-methyladenine from alkylated DNA.

摘要

我们已经确定了编码来自大肠杆菌的3-甲基腺嘌呤DNA糖基化酶I的tag基因的完整核苷酸序列。从核苷酸序列推断，tag酶由187个氨基酸组成，计算分子量为21.1千道尔顿。tag酶的半胱氨酸含量异常丰富（8个残基），在C末端附近有三个连续半胱氨酸组成的簇。尽管tag编码的DNA糖基化酶和alkA编码的糖基化酶都催化从烷基化DNA中释放游离的3-甲基腺嘌呤，但它们之间没有显著的序列同源性。