Suzuki Michiyo, Hattori Yuya, Saito Toshiyuki, Harada Yoshinobu
Takasaki Advanced Radiation Research Institute, National Institutes for Quantum Science and Technology (QST-Takasaki), 1233 Watanuki, Takasaki 370-1292, Gunma, Japan.
National Institute of Radiological Sciences, National Institutes for Quantum Science and Technology (QST-NIRS), 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Chiba, Japan.
Biology (Basel). 2022 Feb 21;11(2):335. doi: 10.3390/biology11020335.
Chemotaxis in the nematode has basically been examined using conventional assay methods. Although these can be problematic, for example, in their use of anesthesia, the method has never been improved. We propose a pond assay for the sensory systems (PASS) of as a novel population-based method of behavioral analysis. The test solution is injected into a recess(es) formed on agar and the response of to its odor and/or taste is examined. Once individuals fall into recesses (ponds) filled with liquid, they cannot return to a solid medium. In this way, the animals are trapped with certainty without the use of anesthesia. The anesthesia used to keep animals in the attractant area in conventional chemotaxis assays is no longer required, allowing pure evaluation of the attractant or repellent response to specific substances. Furthermore, the assay itself can be greatly streamlined because the preparation can be completed simply by providing a recess(es) and filling the liquid. The present paper reports the detailed method and effectiveness of the novel PASS.
线虫的趋化性基本上是使用传统检测方法进行研究的。尽管这些方法可能存在问题，例如在使用麻醉方面，但该方法从未得到改进。我们提出了一种用于线虫感觉系统的池塘检测法（PASS），作为一种基于群体的新型行为分析方法。将测试溶液注入琼脂上形成的凹槽中，然后检测线虫对其气味和/或味道的反应。一旦线虫个体落入充满液体的凹槽（池塘）中，它们就无法回到固体培养基上。通过这种方式，无需使用麻醉就能确切地捕获动物。在传统趋化性检测中用于将动物保持在引诱剂区域的麻醉不再需要，从而可以对特定物质的引诱或驱避反应进行纯粹的评估。此外，该检测本身可以大大简化，因为只需提供一个凹槽并填充液体就能完成准备工作。本文报告了新型PASS的详细方法和有效性。
