Zheng Boying, Han Yuanyuan, Yuan Ruizhong, Liu Jingxian, Tang Pu, van Achterberg Cornelis, Chen Xuexin
State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou 310058, China.
Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
Genes (Basel). 2022 Jan 25;13(2):218. doi: 10.3390/genes13020218.
We obtained four mitochondrial genomes of sp., , and , which represent the first two representatives from subfamily Eucerotinae and Xoridinae (Ichneumonidae), respectively. All of the 4 newly sequenced mitochondrial genomes contain 13 protein-coding genes (PCGs) and most 24 RNA genes. Furthermore, they all have novel tRNA rearrangement patterns comparing with published mitogenomes in Ichneumonidae. For the tRNA cluster , is shuffled as with inversed, while sp. with a remote translocation of , shuffling as . and shared the same cluster . Finally, we reconstructed the phylogenetic relationships among the sequenced subfamilies of Ichneumonidae based on nucleotides and amino acids sequences of 13 PCGs in mitochondrial genomes, and the results of both the maximum likelihood and Bayesian inference analyses highly support that Eucerotinae is the basal ichneumonid lineage rather than Xoridinae.
我们获得了 、 、 和 的四个线粒体基因组，它们分别代表了真角叶蜂亚科和异角叶蜂亚科（姬蜂科）的前两个代表物种。所有这 4 个新测序的线粒体基因组都包含 13 个蛋白质编码基因（PCGs）和大多数 24 个 RNA 基因。此外，与已发表的姬蜂科线粒体基因组相比，它们都具有新颖的 tRNA 重排模式。对于 tRNA 簇 ， 被改组为 ， 发生了倒位，而 物种的 发生了远距离易位，改组为 。 和 共享相同的簇 。最后，我们基于线粒体基因组中 13 个 PCGs 的核苷酸和氨基酸序列重建了姬蜂科测序亚科之间的系统发育关系，最大似然法和贝叶斯推断分析的结果都高度支持真角叶蜂亚科是姬蜂科的基部谱系，而不是异角叶蜂亚科。
