Liu Wenbin, Wang Chengyan, Wang Jingyuan, Tang Yaning, Pei Wenxuan, Ge Xinyu, Yan Chuncai
Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity, Tianjin Normal University, Tianjin 300387, China.
Insects. 2024 Aug 26;15(9):642. doi: 10.3390/insects15090642.
(1) Background: Mitochondrial genomes have been extensively employed as a crucial marker in numerous dipteran families for understanding phylogenetics and systematics relations, thereby playing a pivotal role in molecular biology studies. The phylogenetic relationship of the generic complex remains contentious due to the paucity of taxonomic and molecular data. Specifically, the evolutionary relationships among , , and Harnischia are still unclear. (2) Methods: In this study, and were used as outgroups to analyze phylogenetic relationships among , , and , mitogenomes of four , two , two and two were newly sequenced. Subsequently, we conducted a thorough analysis of the nucleotide composition, sequence length, and evolutionary rate. (3) Results: All mitogenomes exhibited structural conservation, with all genes consistently arranged in the identical order as that of the ancestral mitogenome. Nucleotide composition varied significantly among different genes, and the control region displayed the highest A + T content. All protein-coding genes undergo rigorous purification selection, with the ATP8 gene exhibiting the most rapid evolutionary rate among them. Utilizing Bayesian Inference (BI) and Maximum Likelihood (ML) methods across various databases, we reconstructed the phylogenetic relationships among the genera within the generic complex, drawing insights from an analysis of 14 mitochondrial genomes. (4) Conclusions: Our results showed that the monophyly of the genera was well supported in all topologies; is sister to .
(1) 背景：线粒体基因组已被广泛用作众多双翅目昆虫家族中理解系统发育和系统关系的关键标记，因此在分子生物学研究中发挥着关键作用。由于分类学和分子数据的匮乏，该类属复合体的系统发育关系仍存在争议。具体而言，[具体属名1]、[具体属名2]和哈氏属（Harnischia）之间的进化关系仍不明确。(2) 方法：在本研究中，[外类群属名1]和[外类群属名2]被用作外类群，以分析[具体属名1]、[具体属名2]和[具体属名3]之间的系统发育关系，新测序了4个[具体属名1]、2个[具体属名2]、2个[具体属名3]和2个哈氏属的线粒体基因组。随后，我们对核苷酸组成、序列长度和进化速率进行了全面分析。(3) 结果：所有线粒体基因组均表现出结构保守性，所有基因的排列顺序与祖先线粒体基因组一致。不同基因的核苷酸组成差异显著，控制区的A + T含量最高。所有蛋白质编码基因都经历了严格的纯化选择，其中ATP8基因的进化速率最快。利用贝叶斯推断（BI）和最大似然（ML）方法，通过对14个线粒体基因组的分析，我们重建了该类属复合体中各属之间的系统发育关系。(4) 结论：我们的结果表明，在所有拓扑结构中，[具体属名1]属的单系性都得到了很好的支持；[具体属名2]是[具体属名3]的姐妹群。
