Phylogenetic and Comparative Analysis of , and Inferred from Mitogenomes (Diptera: Chironomidae).

(1) Background: Mitochondrial genomes have been extensively employed as a crucial marker in numerous dipteran families for understanding phylogenetics and systematics relations, thereby playing a pivotal role in molecular biology studies. The phylogenetic relationship of the generic complex remains contentious due to the paucity of taxonomic and molecular data. Specifically, the evolutionary relationships among , , and Harnischia are still unclear. (2) Methods: In this study, and were used as outgroups to analyze phylogenetic relationships among , , and , mitogenomes of four , two , two and two were newly sequenced. Subsequently, we conducted a thorough analysis of the nucleotide composition, sequence length, and evolutionary rate. (3) Results: All mitogenomes exhibited structural conservation, with all genes consistently arranged in the identical order as that of the ancestral mitogenome. Nucleotide composition varied significantly among different genes, and the control region displayed the highest A + T content. All protein-coding genes undergo rigorous purification selection, with the ATP8 gene exhibiting the most rapid evolutionary rate among them. Utilizing Bayesian Inference (BI) and Maximum Likelihood (ML) methods across various databases, we reconstructed the phylogenetic relationships among the genera within the generic complex, drawing insights from an analysis of 14 mitochondrial genomes. (4) Conclusions: Our results showed that the monophyly of the genera was well supported in all topologies; is sister to .