College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Genes (Basel). 2022 Jan 28;13(2):260. doi: 10.3390/genes13020260.
As a centre enzyme in fatty acid activation, acyl-CoA synthetase long-chain family member 1 (ACSL1) plays an important role in body lipid homeostasis. However, the functions of in the subcutaneous adipogenesis of pigs are largely unknown. In the present study, we found that the expression of significantly increased during the process of porcine preadipocyte differentiation. Moreover, silencing of in preadipocytes decreased levels of triglyceride and adipogenic-related markers, including , , and ( < 0.01), and simultaneously increased levels of lipolytic-related markers, such as and ( < 0.05). Conversely, overexpression of in preadipocytes increased levels of triglyceride and , , and ( < 0.01), and reduced levels of and ( < 0.05). Luciferase reporter assays revealed that is a target of miR-218-5p, which can reduce the mRNA and protein levels of by directly binding the 3' untranslated region of . Furthermore, miR-218-5p has an inhibition role in porcine preadipocyte differentiation by suppressing expression. Taken together, these data provide insights into the mechanism of the miR-218-5p/ axis in regulating subcutaneous fat deposition of pigs.
作为脂肪酸激活的中心酶，长链酰基辅酶 A 合成酶家族成员 1（ACSL1）在体内脂质动态平衡中发挥重要作用。然而，其在猪皮下脂肪生成中的功能很大程度上是未知的。在本研究中，我们发现 在猪前体脂肪细胞分化过程中表达显著增加。此外，在脂肪前体细胞中沉默 会降低甘油三酯水平和脂肪生成相关标志物的水平，包括 、 和 （<0.01），同时会增加脂肪分解相关标志物的水平，如 和 （<0.05）。相反，在脂肪前体细胞中过表达 会增加甘油三酯和 、 、 和 （<0.01）的水平，并降低 和 （<0.05）的水平。荧光素酶报告基因检测表明 是 miR-218-5p 的靶标，它可以通过直接结合 的 3'非翻译区来减少 的 mRNA 和蛋白水平。此外，miR-218-5p 通过抑制 表达在猪前体脂肪细胞分化中发挥抑制作用。总之，这些数据为 miR-218-5p/ 轴调节猪皮下脂肪沉积的机制提供了新的见解。
