Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture, Institute ofAnimal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Int J Mol Sci. 2020 Apr 1;21(7):2446. doi: 10.3390/ijms21072446.
Fat deposition, which influences pork production, meat quality and growth efficiency, is an economically important trait in pigs. Numerous studies have demonstrated that stearoyl-CoA desaturase (SCD), a key enzyme that catalyzes the conversion of saturated fatty acids into monounsaturated fatty acids, is associated with fatty acid composition in pigs. As was observed to be significantly induced in 3T3-L1 preadipocytes differentiation, we hypothesized that it plays a role in porcine adipocyte differentiation and fat deposition. In this study, we revealed that is highly expressed in adipose tissues from seven-day-old piglets, compared to its expression in tissues from four-month-old adult pigs. Moreover, we found that and lipogenesis-related genes were induced significantly in differentiated porcine adipocytes. Using CRISPR/Cas9 technology, we generated porcine embryonic fibroblasts (PEFs) and found that the loss of led to dramatically decreased transdifferentiation efficiency, as evidenced by the decreased expression of known lipid synthesis-related genes, lower levels of oil red O staining and significantly lower levels of triglyceride content. Our study demonstrates the critical role of expression in porcine adipocyte differentiation and paves the way for identifying it as the promising candidate gene for less fat deposition in pigs.
脂肪沉积影响猪肉生产、肉质和生长效率，是猪的一个重要经济性状。许多研究表明，硬脂酰辅酶 A 去饱和酶（SCD）是一种将饱和脂肪酸转化为单不饱和脂肪酸的关键酶，与猪的脂肪酸组成有关。由于 在 3T3-L1 前体脂肪细胞分化中被显著诱导，我们假设它在猪脂肪细胞分化和脂肪沉积中发挥作用。在这项研究中，我们揭示了 在 7 日龄仔猪的脂肪组织中高度表达，而在 4 个月大成年猪的组织中表达较低。此外，我们发现 和与脂肪生成相关的基因在分化的猪脂肪细胞中显著诱导。使用 CRISPR/Cas9 技术，我们生成了 的猪胚胎成纤维细胞（PEFs），并发现 的缺失导致明显降低的转分化效率，这表现为已知与脂质合成相关的基因表达降低，油红 O 染色水平降低，甘油三酯含量显著降低。我们的研究表明了 在猪脂肪细胞分化中的关键作用，并为将其确定为减少猪脂肪沉积的有前途的候选基因铺平了道路。
