绵羊羔皮毛囊组织中 microRNAs 和 mRNAs 的整合分析揭示了其形态发生模式

Integrated Hair Follicle Profiles of microRNAs and mRNAs to Reveal the Pattern Formation of Hu Sheep Lambskin.

机构信息

Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.

College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.

出版信息

Genes (Basel). 2022 Feb 14;13(2):342. doi: 10.3390/genes13020342.

Hair follicle development is closely associated with wool curvature. Current studies reveal the crucial role of microRNAs (miRNAs) in hair follicle growth and development. However, few studies are known regarding their role in wool curvature. To reveal the potential roles of miRNAs in Hu sheep lambskin with different patterns, a total of 37 differentially expressed (DE) miRNAs were identified in hair follicles between small waves (SM) and straight wool (ST) groups using RNA-seq. Through functional enrichment and miRNA-mRNA co-expression analysis, some key miRNAs (oar-miR-143, oar-miR-200b, oar-miR-10a, oar-miR-181a, oar-miR-10b, oar-miR-125b, etc.) and miRNA-mRNA pairs (miR-125b target , miR-181a target , , miR-200b target , etc.) were identified. Though direct or indirect ways affecting hair follicle development, these miRNAs and mRNAs may have possible effects on wool curvature, and this study thus provides valuable insight on potential pattern formation.

毛囊发育与羊毛卷曲密切相关。目前的研究揭示了 microRNAs（miRNAs）在毛囊生长和发育中的关键作用。然而，关于它们在羊毛卷曲中的作用知之甚少。为了揭示 miRNA 在不同图案的湖羊羔羊皮中的潜在作用，使用 RNA-seq 在小波浪（SM）和直羊毛（ST）组之间的毛囊中鉴定了总共 37 个差异表达（DE）miRNAs。通过功能富集和 miRNA-mRNA 共表达分析，鉴定了一些关键的 miRNA（oar-miR-143、oar-miR-200b、oar-miR-10a、oar-miR-181a、oar-miR-10b、oar-miR-125b 等）和 miRNA-mRNA 对（miR-125b 靶标、miR-181a 靶标、、miR-200b 靶标等）。这些 miRNA 和 mRNAs 可能通过直接或间接影响毛囊发育，对羊毛卷曲产生潜在影响，因此本研究为潜在的图案形成提供了有价值的见解。