Huebner K, ar-Rushdi A, Griffin C A, Isobe M, Kozak C, Emanuel B S, Nagarajan L, Cleveland J L, Bonner T I, Goldsborough M D
Proc Natl Acad Sci U S A. 1986 Jun;83(11):3934-8. doi: 10.1073/pnas.83.11.3934.
Murine and human cDNAs, related to but distinct from c-raf-1, have been isolated and designated mA-raf and hA-raf, respectively. The mA-raf and hA-raf cDNAs detect the same murine and human fragments in Southern blots of restriction enzyme-cleaved murine and human cellular DNA. The murine restriction enzyme fragments homologous to mA-raf cDNA cosegregate with mouse chromosome X in a panel of Chinese hamster-mouse hybrid cells, thus localizing the mA-raf locus to mouse chromosome X. Two independently segregating loci, detected by the hA-raf cDNA (or mA-raf cDNA), hA-raf-1 and hA-raf-2, are located on human chromosomes X and 7, respectively. The mA-raf locus and the hA-raf-1 locus are actively transcribed in several mouse and human cell lines.
已分离出与c-raf-1相关但又不同的小鼠和人类cDNA，分别命名为mA-raf和hA-raf。在经限制性内切酶切割的小鼠和人类细胞DNA的Southern印迹中，mA-raf和hA-raf cDNA能检测到相同的小鼠和人类片段。与mA-raf cDNA同源的小鼠限制性内切酶片段，在一组中国仓鼠-小鼠杂交细胞中与小鼠X染色体共分离，从而将mA-raf基因座定位到小鼠X染色体上。由hA-raf cDNA（或mA-raf cDNA）检测到的两个独立分离的基因座，hA-raf-1和hA-raf-2，分别位于人类染色体X和7上。mA-raf基因座和hA-raf-1基因座在几种小鼠和人类细胞系中均有活跃转录。
