Irani A A, Schechter N M, Craig S S, DeBlois G, Schwartz L B
Proc Natl Acad Sci U S A. 1986 Jun;83(12):4464-8. doi: 10.1073/pnas.83.12.4464.
Two human mast cell types were identified by immunohistochemical techniques in skin, lung, and small intestine. One type contains the neutral proteases, tryptase and chymotryptic proteinase, and is termed the TC mast cell. The second type contains only tryptase and is termed the T mast cell. Both types are fixed better by Carnoy's fluid than by formalin. The percentage of mast cells accounted for by the T type was 12 in skin; 98 in mucosa and 13 in submucosa of small intestine; and 77 in bronchial/bronchiolar subepithelium, about 97 in bronchial/bronchiolar epithelium, and 93 in alveoli of lung. Dispersed lung cells contained 90% T mast cells. The mean area of TC mast cells (76 micron2) was slightly larger than that of T mast cells (66 micron2); however, there was such extensive overlap that individual mast cells belonging to different types could not be distinguished on the basis of size. The recognition of human mast cell types with distinct protease compositions suggests a higher level of complexity of human mast cell-mediated reactions than heretofore appreciated.
通过免疫组织化学技术在皮肤、肺和小肠中鉴定出两种人类肥大细胞类型。一种类型含有中性蛋白酶、类胰蛋白酶和糜蛋白酶，被称为TC肥大细胞。第二种类型仅含类胰蛋白酶，被称为T肥大细胞。这两种类型用卡诺氏固定液固定比用福尔马林固定效果更好。T型肥大细胞在皮肤中占比为12%；在小肠黏膜中占98%，在黏膜下层占13%；在支气管/细支气管上皮下占77%，在支气管/细支气管上皮中约占97%，在肺肺泡中占93%。分散的肺细胞含90%的T肥大细胞。TC肥大细胞的平均面积（76平方微米）略大于T肥大细胞（66平方微米）；然而，两者有大量重叠，以至于无法根据大小区分不同类型的单个肥大细胞。对具有不同蛋白酶组成的人类肥大细胞类型的识别表明，人类肥大细胞介导的反应比以往认识到的更为复杂。
