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GlSwi6通过细胞内钙信号在……中正向调节纤维素酶和木聚糖酶活性 。 （原文句末不完整，翻译时根据已有内容尽量准确表达）

GlSwi6 Positively Regulates Cellulase and Xylanase Activities through Intracellular Ca Signaling in .

作者信息

Lian Ling-Dan, Shi Ling-Yan, Zhu Jing, Liu Rui, Shi Liang, Ren Ang, Yu Han-Shou, Zhao Ming-Wen

机构信息

Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.

出版信息

J Fungi (Basel). 2022 Feb 14;8(2):187. doi: 10.3390/jof8020187.

DOI:10.3390/jof8020187
PMID:35205940
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8877461/
Abstract

is a white-rot fungus that produces a range of lignocellulolytic enzymes to decompose lignin and cellulose. The mitogen-activated protein kinase (MAPK) pathway has been implicated in xylanases and cellulases production. As the downstream transcription factor of Slt2-MAPK, the function of Swi6 in has not been fully studied. In this study, the transcription factor GlSwi6 in was characterized and shown to significantly positively regulate cellulases and xylanases production. Knockdown of the GlSwi6 gene decreased the activities of cellulases and xylanases by approximately 31%38% and 54%60% compared with those of the wild-type (WT) strain, respectively. Besides, GlSwi6 can be alternatively spliced into two isoforms, GlSwi6A and GlSwi6B, and overexpression of GlSwi6B increased the activities of cellulase and xylanase by approximately 50% and 60%, respectively. Further study indicates that the existence of GlSwi6B significantly increased the concentration of cytosolic Ca. Our study indicated that GlSwi6 promotes the activities of cellulase and xylanase by regulating the Ca signaling. These results connected the GlSwi6 and Ca signaling in the regulation of cellulose degradation, and provide an insight for further improvement of cellulase or xylanase activities in as well as other fungi.

摘要

是一种白腐真菌，可产生一系列木质纤维素分解酶以分解木质素和纤维素。丝裂原活化蛋白激酶（MAPK）途径与木聚糖酶和纤维素酶的产生有关。作为Slt2-MAPK的下游转录因子，Swi6在其中的功能尚未得到充分研究。在本研究中，对中的转录因子GlSwi6进行了表征，并表明其对纤维素酶和木聚糖酶的产生具有显著的正向调节作用。与野生型（WT）菌株相比，敲低GlSwi6基因后，纤维素酶和木聚糖酶的活性分别降低了约31%38%和54%60%。此外，GlSwi6可选择性剪接为两种异构体GlSwi6A和GlSwi6B，过表达GlSwi6B可使纤维素酶和木聚糖酶的活性分别提高约50%和60%。进一步研究表明，GlSwi6B的存在显著提高了胞质Ca的浓度。我们的研究表明，GlSwi6通过调节Ca信号促进纤维素酶和木聚糖酶的活性。这些结果将GlSwi6与Ca信号联系起来，参与纤维素降解的调控，并为进一步提高中的纤维素酶或木聚糖酶活性以及其他真菌提供了思路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d1a/8877461/0bee9f4375c8/jof-08-00187-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d1a/8877461/05d4a29e59b9/jof-08-00187-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d1a/8877461/4de115b876be/jof-08-00187-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d1a/8877461/1ba860e0db4a/jof-08-00187-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d1a/8877461/15ada9ef13fd/jof-08-00187-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d1a/8877461/0bee9f4375c8/jof-08-00187-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d1a/8877461/05d4a29e59b9/jof-08-00187-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d1a/8877461/4de115b876be/jof-08-00187-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d1a/8877461/1ba860e0db4a/jof-08-00187-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d1a/8877461/15ada9ef13fd/jof-08-00187-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d1a/8877461/0bee9f4375c8/jof-08-00187-g005.jpg

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