Molecular Biotechnology Laboratory, Department of Biochemistry and Immunology, Ribeirao Preto Medical School (FMRP), University of Sao Paulo, Ribeirao Preto, SP, Brazil.
Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Campinas, SP, Brazil.
Sci Rep. 2018 Oct 8;8(1):14931. doi: 10.1038/s41598-018-33383-1.
In this study, through global transcriptional analysis by RNA-Sequencing, we identified the main changes in gene expression that occurred in two functional mutants of the MAPK genes tmk1 and tmk2 in Trichoderma reesei during sugarcane bagasse degradation. We found that the proteins encoded by these genes regulated independent processes, sometimes in a cross-talk manner, to modulate gene expression in T. reesei. In the Δtmk2 strain, growth in sugarcane bagasse modulated the expression of genes involved in carbohydrate metabolism, cell growth and development, and G-protein-coupled receptor-mediated cell signaling. On the other hand, deletion of tmk1 led to decreased expression of the major genes for cellulases and xylanases. Furthermore, TMK1 found to be involved in the regulation of the expression of major facilitator superfamily transporters. Our results revealed that the MAPK signaling pathway in T. reesei regulates many important processes that allow the fungus to recognize, transport, and metabolize different carbon sources during plant cell wall degradation.
在这项研究中，通过 RNA 测序的全转录组分析，我们确定了在甘蔗渣降解过程中，里氏木霉 MAPK 基因 tmk1 和 tmk2 的两个功能突变体中发生的主要基因表达变化。我们发现，这些基因编码的蛋白质调节独立的过程，有时以串扰的方式调节里氏木霉中的基因表达。在Δtmk2 菌株中，在甘蔗渣上的生长调节了参与碳水化合物代谢、细胞生长和发育以及 G 蛋白偶联受体介导的细胞信号转导的基因的表达。另一方面，tmk1 的缺失导致纤维素酶和木聚糖酶的主要基因表达降低。此外，发现 TMK1 参与主要易化剂超家族转运蛋白表达的调节。我们的结果表明，里氏木霉中的 MAPK 信号通路调节许多重要过程，使真菌能够在植物细胞壁降解过程中识别、运输和代谢不同的碳源。
