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对黑 chokeberry（黑果腺肋花楸）、蔓越莓和石榴提取物进行分级分离，以鉴定影响脂质代谢的化合物。

Fractionation of Extracts from Black Chokeberry, Cranberry, and Pomegranate to Identify Compounds That Influence Lipid Metabolism.

作者信息

Niesen Sonja, Göttel Celina, Becker Hanna, Bakuradze Tamara, Winterhalter Peter, Richling Elke

机构信息

Institute of Food Chemistry, Technische Universität Braunschweig, Schleinitzstraße 20, D-38106 Braunschweig, Germany.

Division of Food Chemistry and Toxicology, Department of Chemistry, Technische Universität Kaiserslautern, Erwin-Schrödinger-Straße 52, D-67663 Kaiserslautern, Germany.

出版信息

Foods. 2022 Feb 16;11(4):570. doi: 10.3390/foods11040570.

DOI:10.3390/foods11040570
PMID:35206045
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8871205/
Abstract

Polyphenols show a spectrum of bioactive effects, including an influence on lipid metabolism. In this study, we performed activity-guided fractionations of black chokeberry (aronia), cranberry, and pomegranate extracts to identify the biologically active compounds. The extracts were prepared from fruit juice concentrates with the adsorbent resin Amberlite XAD-7 and were separated into a copigment and an anthocyanin fraction, followed by fractionation into a polymer and monomeric fraction by means of hexane precipitation. For further fractionation of the cranberry and pomegranate copigment fractions, high-performance countercurrent chromatography (HPCCC) was used. The compounds in each fraction were identified by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS), and the quantification was performed by ultra high-performance liquid chromatography-diode array detector (UHPLC-DAD) analyses. Each of the (sub-)fractions was tested in three in vitro assays: phosphodiesterase 3B (PDE) activity, lipid accumulation, and lipolysis in 3T3-L1 cells. The results showed that various fractions and subfractions can inhibit lipid accumulation and PDE activity as well as increase lipolysis, particularly copigments. Overall, our results indicate an influence of polyphenol-rich (sub-)fractions on the lipid metabolism.

摘要

多酚具有一系列生物活性作用，包括对脂质代谢的影响。在本研究中，我们对黑接骨木莓（黑果腺肋花楸）、蔓越莓和石榴提取物进行了活性导向分级分离，以鉴定生物活性化合物。提取物由果汁浓缩物与吸附树脂Amberlite XAD - 7制备，先分离为共色素和花青素级分，然后通过己烷沉淀进一步分离为聚合物级分和单体级分。对于蔓越莓和石榴共色素级分的进一步分级分离，采用了高效逆流色谱法（HPCCC）。通过高效液相色谱/电喷雾电离串联质谱法（HPLC - ESI - MS/MS）鉴定各馏分中的化合物，并通过超高效液相色谱 - 二极管阵列检测器（UHPLC - DAD）分析进行定量。每个（亚）馏分在三种体外试验中进行测试：3T3 - L1细胞中的磷酸二酯酶3B（PDE）活性、脂质积累和脂肪分解。结果表明，各种馏分和亚馏分可抑制脂质积累和PDE活性，并增加脂肪分解，尤其是共色素。总体而言，我们的结果表明富含多酚的（亚）馏分对脂质代谢有影响。

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