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[宋内志贺菌I期、II期及R型的鉴别特征]

[Differential characteristics of the I and II phases and R-form of Sh. sonnei].

作者信息

Nastichkin I A, Miroliubova L V

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1978 May(5):16-20.

PMID:352062
Abstract

Characteristics of various phases of Sh. sonnei were determined on the standard strains of Sh. sonnei of phases I and II and R-form used for the industrial production of agglutinating monoreceptor sera. Bacteria of phase I displayed a distinct morphological, cultural and serological differences. For the differentiation of Sh. sonnei of the II phase and R-form, representing the greatest difficulty in this respect, it is recommended to use phages RFfm and 6-SR, and also indirect immunofluorescence method with the application of agglutinating monophasic serum against the II phase of Sh. sonnei. In addition, a study was made of over 20 various Sh. sonnei strains at different dissociative process phases. Verification data completely confirmed the specificity of phages RFfm and 6-SR for Sh. sonnei in the II phase. The efficacy of the immunofluorescence method was confirmed on 6 strains.

摘要

在用于工业生产凝集单受体血清的I相和II相宋内志贺氏菌标准菌株及R型菌株上,测定了宋内志贺氏菌各阶段的特征。I相细菌表现出明显的形态、培养和血清学差异。对于II相宋内志贺氏菌和R型菌的鉴别,这在这方面是最大的困难,建议使用噬菌体RFfm和6-SR,以及应用针对宋内志贺氏菌II相的凝集单相血清的间接免疫荧光法。此外,对20多种处于不同解离过程阶段的宋内志贺氏菌菌株进行了研究。验证数据完全证实了噬菌体RFfm和6-SR对II相宋内志贺氏菌的特异性。免疫荧光法在6株菌株上得到了证实。

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