Nastichkin I A, Lycheva T A, Petrovskaia V G
Zh Mikrobiol Epidemiol Immunobiol. 1981 Dec(12):82-7.
The possibility of obtaining Sh. sonnei donor strains in phases I and II by two methods is shown: by the transfer of the distal market lac+ from E. coli K12, Hfr-strain P4X, or by using F'ts lac+ episome. Of 5 Sh. sonnei donor strains in phase I, obtained by hybridization with E. coli K12, Hfr-strain P4X, 3 strains retained their virulence, in contrast to Sh. flexneri donor strains in the S-form, previously obtained by similar hybrizations. Of 8 Sh. sonnei donor strains in phase I, obtained by hybrididization with E. coli K12 KS 881, 6 strains were virulent. Sh. sonnei donor strains in phases I and II obtained in these experiments lost their fertility after being kept on the Dorset egg medium at 4 degrees C for 1.5--2 months.
通过从大肠杆菌K12、高频重组菌株P4X转移远端市场lac⁺，或使用F'ts lac⁺附加体。在通过与大肠杆菌K12、高频重组菌株P4X杂交获得的5株第一阶段宋内志贺氏菌供体菌株中，有3株保留了它们的毒力，这与之前通过类似杂交获得的S型福氏志贺氏菌供体菌株形成对比。在通过与大肠杆菌K12 KS 881杂交获得的8株第一阶段宋内志贺氏菌供体菌株中，有6株具有毒力。在这些实验中获得的第一阶段和第二阶段宋内志贺氏菌供体菌株在4℃的多塞特卵培养基上保存1.5 - 2个月后失去了育性。
