[宋内志贺氏菌供体菌株的分离与特性]

[Isolation and characteristics of Shigella sonnei donor strains].

作者信息

Nastichkin I A, Lycheva T A, Petrovskaia V G

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1981 Dec(12):82-7.

Abstract

The possibility of obtaining Sh. sonnei donor strains in phases I and II by two methods is shown: by the transfer of the distal market lac+ from E. coli K12, Hfr-strain P4X, or by using F'ts lac+ episome. Of 5 Sh. sonnei donor strains in phase I, obtained by hybridization with E. coli K12, Hfr-strain P4X, 3 strains retained their virulence, in contrast to Sh. flexneri donor strains in the S-form, previously obtained by similar hybrizations. Of 8 Sh. sonnei donor strains in phase I, obtained by hybrididization with E. coli K12 KS 881, 6 strains were virulent. Sh. sonnei donor strains in phases I and II obtained in these experiments lost their fertility after being kept on the Dorset egg medium at 4 degrees C for 1.5--2 months.

摘要

展示了通过两种方法在第一阶段和第二阶段获得宋内志贺氏菌供体菌株的可能性

通过从大肠杆菌K12、高频重组菌株P4X转移远端市场lac⁺，或使用F'ts lac⁺附加体。在通过与大肠杆菌K12、高频重组菌株P4X杂交获得的5株第一阶段宋内志贺氏菌供体菌株中，有3株保留了它们的毒力，这与之前通过类似杂交获得的S型福氏志贺氏菌供体菌株形成对比。在通过与大肠杆菌K12 KS 881杂交获得的8株第一阶段宋内志贺氏菌供体菌株中，有6株具有毒力。在这些实验中获得的第一阶段和第二阶段宋内志贺氏菌供体菌株在4℃的多塞特卵培养基上保存1.5 - 2个月后失去了育性。