Department of Water Protection Engineering and Environmental Microbiology, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1, 10-720 Olsztyn, Poland.
Department of Ichthyology and Aquaculture, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-719 Olsztyn, Poland.
Int J Environ Res Public Health. 2022 Feb 18;19(4):2379. doi: 10.3390/ijerph19042379.
Human activities influence the presence of potentially pathogenic bacteria in indoor air. The aim of this study was to determine the effect of the experimental rearing of European grayling and European perch in a recirculating aquaculture system on the contamination of indoor air with potentially pathogenic (PPAH) and the resulting health risks to humans. The PPAH counts, their resistance to seven antibiotics, and the multiple antibiotic resistance (MAR) index were determined in samples of indoor air and water from rearing tanks. The PPAH counts were highest in the laboratory bioaerosol where two fish species were reared. The calculated indoor/outdoor ratio (I/O > 1) demonstrated that tank water was the internal source of PPAH emissions. The unconstrained PCA revealed strong positive relationships ( ≤ 0.05) between the PPAH counts in the indoor air and water samples. Most of the PPAH strains isolated from laboratory air were resistant to tetracycline, cefotaxime, and erythromycin, and 26-82% of the isolates exhibited multiple drug resistance. The values of the MAR index were similar in samples of laboratory air and water (0.23-0.34 and 0.24-0.36, respectively). Agglomerative clustering revealed two clusters of strains isolated from laboratory air and tank water. The results of this study indicate that aquaculture can be a source of indoor air contamination with PPAH.
人类活动会影响室内空气中潜在致病细菌的存在。本研究旨在确定在循环水产养殖系统中人工养殖欧洲山红点鲑和欧洲鲈鱼对室内空气中潜在致病细菌（PPAH）污染的影响，以及对人类健康造成的潜在风险。对养殖水箱内的室内空气和水样本中的 PPAH 计数、它们对七种抗生素的耐药性和多重抗生素耐药性（MAR）指数进行了测定。在养殖两种鱼类的实验室生物气溶胶中，PPAH 计数最高。计算得出的室内/室外比值（I/O＞1）表明，水箱水是 PPAH 排放的内部来源。无约束主成分分析显示，室内空气和水样中的 PPAH 计数之间存在很强的正相关关系（≤0.05）。从实验室空气中分离出的大多数 PPAH 菌株对四环素、头孢噻肟和红霉素具有耐药性，26-82%的分离株表现出多重耐药性。实验室空气和水样中 MAR 指数的值相似（分别为 0.23-0.34 和 0.24-0.36）。凝聚聚类显示，从实验室空气和水箱水中分离出的菌株分为两个群。本研究结果表明，水产养殖可能是室内空气 PPAH 污染的一个来源。
