College of Resource and Environment Science, Nanjing Agricultural University, Nanjing 210095, China.
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Int J Environ Res Public Health. 2022 Feb 19;19(4):2416. doi: 10.3390/ijerph19042416.
Environmental exposure to cadmium (Cd) contributes to a decline in the quality of human semen. Although the testis is sensitive to Cd exposure, the mechanism underlying how cadmium affects the testis remains to be defined. In this study, male mice were treated with intraperitoneal injections of 0, 0.5, 1.5 and 2.5 mg CdCl/kg/day for 10 days, respectively. Both the testicular weight and the 3β-HSD activity of Leydig cells were significantly reduced with the administration of 2.5 mg CdCl/kg/day. The height of endothelial cells in the interstitial blood vessels significantly increased with the use of 2.5 mg CdCl/kg/day compared with the control. Western blot data showed that the protein levels of CD31, αSMA, caveolin and Ng2 increased with cadmium exposure, and this increase was particularly significant with the administration of 2.5 mg CdCl/kg/day. CD31, αSMA, caveolin and Ng2 are related to angiogenesis. Based on our data, cadmium exposure may stimulate the proliferation of the mural cells and endothelial cells of blood vessels, which may lead to abnormal function of the testis.
环境镉（Cd）暴露会导致人类精液质量下降。尽管睾丸对 Cd 暴露敏感，但 Cd 如何影响睾丸的机制仍有待确定。在这项研究中，雄性小鼠分别接受腹腔注射 0、0.5、1.5 和 2.5 mg CdCl/kg/天，连续 10 天。与对照组相比，2.5mg CdCl/kg/天组的睾丸重量和间质血管中黄体生成细胞的 3β-HSD 活性明显降低。与对照组相比，2.5mg CdCl/kg/天组的血管间内皮细胞高度明显增加。Western blot 数据显示，随着 Cd 暴露，CD31、αSMA、 caveolin 和 Ng2 的蛋白水平增加，而 2.5mg CdCl/kg/天组的增加尤为显著。CD31、αSMA、 caveolin 和 Ng2 与血管生成有关。基于我们的数据，Cd 暴露可能会刺激血管壁细胞和内皮细胞的增殖，这可能导致睾丸功能异常。
