Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, P.M.B. 1115, Calabar, Cross River State, Nigeria; Department of Physiology, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.
Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, P.M.B. 1115, Calabar, Cross River State, Nigeria.
Biomed Pharmacother. 2017 Oct;94:109-123. doi: 10.1016/j.biopha.2017.07.087. Epub 2017 Jul 27.
This study assessed the effect of quercetin (QE) on cadmium chloride (CdCl) - induced testicular toxicity, as well as the effect of withdrawal of CdCl treatment on same. Thirty male Wistar rats aged 10 weeks old and weighing 270-300g were assigned into 5 groups and used for this study. Rats in groups 1-4 were administered vehicle, CdCl (5mg/kg bwt), CdCl+QE (5mg/kg bwt and 20mg/kg bwt, respectively) or QE (20mg/kg bwt) orally for 4 weeks. Group 5 rats received CdCl, with 4 weeks recovery period. Results showed that cadmium accumulated in serum, testis and epididymis, decreased body weight, testicular and epididymal weights, sperm count, motility and viability. Cadmium decreased serum concentrations of reproductive hormones, but increased testicular glucose, lactate and lactate dehydrogenase activity. Cadmium decreased testicular enzymatic (superoxide dismutase, catalase and glutathione peroxidase) and non-enzymatic (glutathione, vitamins C and E) antioxidants, and increased malondialdehyde and hydrogen peroxide. Cadmium down-regulated Bcl-2 protein, up-regulated Bax protein, increased Bax/Bcl-2 ratio and cleaved caspase-3 activity. Histopathology of the testis showed decreased Johnsen's score and Leydig cell count. These negative effects were attenuated by QE administration, while withdrawal of CdCl did not appreciably reverse toxicity. We conclude that QE better protected the testis from CdCl toxicity than withdrawal of CdCl administration.
本研究评估了槲皮素（QE）对氯化镉（CdCl）诱导的睾丸毒性的影响，以及停止 CdCl 处理对相同毒性的影响。将 30 只 10 周龄、体重 270-300g 的雄性 Wistar 大鼠分为 5 组用于本研究。第 1-4 组大鼠分别给予 vehicle、CdCl（5mg/kg bwt）、CdCl+QE（5mg/kg bwt 和 20mg/kg bwt）或 QE（20mg/kg bwt）口服 4 周。第 5 组大鼠接受 CdCl 处理，并在第 4 周恢复期。结果表明，镉在血清、睾丸和附睾中积累，体重、睾丸和附睾重量、精子计数、活力和活力下降。镉降低了血清生殖激素浓度，但增加了睾丸葡萄糖、乳酸和乳酸脱氢酶活性。镉降低了睾丸酶（超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶）和非酶（谷胱甘肽、维生素 C 和 E）抗氧化剂的浓度，增加了丙二醛和过氧化氢的浓度。镉下调 Bcl-2 蛋白，上调 Bax 蛋白，增加 Bax/Bcl-2 比值和激活 caspase-3。睾丸组织病理学显示约翰森评分和间质细胞计数降低。QE 给药减轻了这些负面影响，而停止 CdCl 处理并没有显著逆转毒性。我们得出结论，QE 比停止 CdCl 处理更能保护睾丸免受 CdCl 毒性。
