Gong Ming-Fu, Yang Xi-Bin, Long Gui-Yun, Jia Ze-Yan, Zeng Qing-Hui, Jin Dao-Chao, Yang Hong, Zhou Cao
Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550025, China.
College of Life Sciences, Chongqing Normal University, Chongqing 401331, China.
Insects. 2022 Feb 6;13(2):174. doi: 10.3390/insects13020174.
The juvenile hormone (JH) is crucial for insect reproduction, and isopentenyl pyrophosphate isomerase (IPPI) is a key enzyme in the JH synthesis pathway. However, few studies have investigated how IPPI regulates insect reproduction. This study identifies and characterizes the IPPI gene () from the important agricultural pest . A phylogenetic analysis reveals a high homology of with the amino acid sequences of and (Stål). Furthermore, is expressed at various developmental stages and in various tissues of and is significantly higher on the 5th day of adult emergence and in integument tissue, while lower levels are found on the 3rd day of adult emergence and in fat body and gut tissue. After silencing using RNA interference, the ovarian development is significantly inhibited and the fecundity is significantly reduced when compared with the control group. Additionally, silencing significantly decreases the expression levels of downstream JH signal transduction pathway genes (, , and ) and . Our findings are helpful in elucidating the molecular mechanism underlying the regulation of insect reproduction through genes in the JH synthesis pathway, and they provide a theoretical basis for the development of pest control treatments targeting .
保幼激素（JH）对昆虫繁殖至关重要，异戊烯基焦磷酸异构酶（IPPI）是JH合成途径中的关键酶。然而，很少有研究探讨IPPI如何调节昆虫繁殖。本研究从重要农业害虫[害虫名称未给出]中鉴定并表征了IPPI基因（[基因名称未给出]）。系统发育分析表明，[该基因名称未给出]与[其他两种昆虫名称未给出]（Stål）的氨基酸序列具有高度同源性。此外，[该基因名称未给出]在[害虫名称未给出]的各个发育阶段和各种组织中均有表达，在成虫羽化第5天和体壁组织中的表达显著较高，而在成虫羽化第3天以及脂肪体和肠道组织中的表达水平较低。使用RNA干扰沉默[该基因名称未给出]后，与对照组相比，卵巢发育受到显著抑制，繁殖力显著降低。此外，沉默[该基因名称未给出]显著降低了下游JH信号转导途径基因（[基因名称未给出]、[基因名称未给出]和[基因名称未给出]）以及[基因名称未给出]的表达水平。我们的研究结果有助于阐明通过JH合成途径中的基因调控昆虫繁殖的分子机制，并为开发针对[害虫名称未给出]的害虫防治方法提供理论依据。
