Jiang Jianru, Xu Yili, Lin Xinda
College of Life Sciences, China Jiliang University, Hangzhou, China.
Front Physiol. 2017 Dec 6;8:1013. doi: 10.3389/fphys.2017.01013. eCollection 2017.
Ovarian development plays an important role in the life history of insects and is crucial for control of the insect population. The metamorphosis of an insect is precisely regulated by the interaction of the juvenile hormone and ecdysone. To understand the role of and in ovary development, we used RNA interference (RNAi) to down-regulate the expression of () and (), two important down-stream transcription factors of juvenile hormone and ecdysone signaling. We further investigated their effects on metamorphosis and ovary development. The results showed that both and are induced by ecdysone. The down-regulation of and alone or together by RNAi is more effective than the topical application of ecdysone on the number of ovarioles, suggesting the necessity of and in determining the number of ovarioles. The ovarian grade was significantly increased/decreased by the topical application of ecdysone and down-regulation of and . The pre-oviposition period was also increased. When and were down-regulated together, the ovary grade was not significantly different compared to the control (dsGFP), indicating that the development of the ovary is under the control of both and . The interaction between the and on the number of ovarioles and the development of the ovary indicates cross-talk between both juvenile hormone and ecdysone signaling at the transcription level in the brown planthopper. Both genes are nuclear transcription factors and may regulate signaling via down-stream genes. These results would help to both enhance the current understanding of the regulatory mechanism of the interaction between juvenile hormone and ecdysone signaling pathways during ovarian development and to design chemicals to control pests.
卵巢发育在昆虫的生活史中起着重要作用，对于控制昆虫种群至关重要。昆虫的变态发育由保幼激素和蜕皮激素的相互作用精确调控。为了了解[具体基因名称1]和[具体基因名称2]在卵巢发育中的作用，我们使用RNA干扰（RNAi）下调了保幼激素和蜕皮激素信号传导的两个重要下游转录因子[具体基因名称1]和[具体基因名称2]的表达。我们进一步研究了它们对变态发育和卵巢发育的影响。结果表明，[具体基因名称1]和[具体基因名称2]均由蜕皮激素诱导。单独或共同通过RNAi下调[具体基因名称1]和[具体基因名称2]，在卵巢小管数量上比局部应用蜕皮激素更有效，这表明[具体基因名称1]和[具体基因名称2]在确定卵巢小管数量方面的必要性。通过局部应用蜕皮激素以及下调[具体基因名称1]和[具体基因名称2]，卵巢等级显著升高/降低。产卵前期也延长了。当同时下调[具体基因名称1]和[具体基因名称2]时，卵巢等级与对照（dsGFP）相比无显著差异，表明卵巢发育受[具体基因名称1]和[具体基因名称2]两者的控制。[具体基因名称1]和[具体基因名称2]在卵巢小管数量和卵巢发育方面的相互作用表明，在褐飞虱转录水平上，保幼激素和蜕皮激素信号传导之间存在相互作用。这两个基因都是核转录因子，可能通过下游基因调节信号传导。这些结果将有助于增进目前对卵巢发育过程中保幼激素和蜕皮激素信号通路相互作用调控机制的理解，并有助于设计控制害虫的化学药剂。
